ENH: add action to get feature lengths

q2_moshpit/__init__.py

@@ -19,12 +19,14 @@
     helpers as kraken_helpers
 )
 from .metabat2 import metabat2
+from ._utils import get_feature_lengths
 
 __version__ = get_versions()['version']
 del get_versions
 
 __all__ = [
     'metabat2', 'bracken', 'kraken_class', 'kraken_db',
     'kaiju_class', 'kaiju_db', 'dereplicate_mags', 'eggnog',
-    'busco', 'prodigal', 'kraken_helpers', 'partition'
+    'busco', 'prodigal', 'kraken_helpers', 'partition',
+    'get_feature_lengths'
 ]

q2_moshpit/_utils.py

@@ -9,6 +9,11 @@
 import hashlib
 from typing import List
 
+import pandas as pd
+import skbio
+
+from q2_types.feature_data_mag import MAGSequencesDirFmt
+
 
 def run_command(cmd, env=None, verbose=True, pipe=False, **kwargs):
     if verbose:
@@ -84,3 +89,16 @@ def _calculate_md5_from_file(file_path: str) -> str:
         for chunk in iter(lambda: f.read(4096), b""):
             md5_hash.update(chunk)
     return md5_hash.hexdigest()
+
+
+def get_feature_lengths(features: MAGSequencesDirFmt) -> pd.DataFrame:
+    """Calculate lengths of features in a feature data object."""
+    ids, lengths = [], []
+    for _id, fp in features.feature_dict().items():
+        sequences = skbio.io.read(fp, format='fasta', verify=False)
+        ids.append(_id)
+        lengths.append(sum(len(seq) for seq in sequences))
+
+    df = pd.DataFrame({'id': ids, 'length': lengths})
+    df.set_index('id', inplace=True)
+    return df

q2_moshpit/plugin_setup.py

@@ -12,7 +12,7 @@
 )
 from q2_types.distance_matrix import DistanceMatrix
 from q2_types.feature_data import (
-    FeatureData, Sequence, Taxonomy, ProteinSequence
+    FeatureData, Sequence, Taxonomy, ProteinSequence, SequenceCharacteristics
 )
 from q2_types.feature_table import FeatureTable, Frequency, PresenceAbsence
 from q2_types.per_sample_sequences import (
@@ -1194,6 +1194,26 @@
     citations=[citations["menzel2016"]],
 )
 
+plugin.methods.register_function(
+    function=q2_moshpit._utils.get_feature_lengths,
+    inputs={
+        "features": FeatureData[MAG],
+    },
+    parameters={},
+    outputs=[
+        ('lengths',
+         FeatureData[SequenceCharacteristics % Properties('length')])
+    ],
+    input_descriptions={
+        "features": "Features to get lengths for."
+    },
+    parameter_descriptions={},
+    output_descriptions={'lengths': 'Feature lengths.', },
+    name='Get feature lengths.',
+    description='This method extract lengths for the provided feature set.',
+    citations=[]
+)
+
 plugin.register_semantic_types(BUSCOResults)
 plugin.register_semantic_type_to_format(
     BUSCOResults,

q2_moshpit/tests/data/mags-derep/24dee6fe-9b84-45bb-8145-de7b092533a1.fasta

@@ -0,0 +1,4 @@
+>NZ_00000000.1_contig1
+ATGTTTCCAGATGCAATGCGTGGGCACTG
+>NZ_00000000.1_contig2
+TTGACGTCAGTGAAAACCATGCAGTGTTGACGTCAGT

q2_moshpit/tests/data/mags-derep/ca7012fc-ba65-40c3-84f5-05aa478a7585.fasta

@@ -0,0 +1,6 @@
+>NZ_CP007255.1_contig1
+GGCGTGCCCTCA
+>NZ_CP007255.1_contig2
+GGAACGAACTCGGTCCAGGCGTTCTCCCACAT
+>NZ_CP007255.1_contig3
+ATGATCGATCCTTCCCACCAAGCTCA

q2_moshpit/tests/data/mags-derep/d65a71fa-4279-4588-b937-0747ed5d604d.fasta

@@ -0,0 +1,11 @@
+>NZ_CP018863.1_contig1
+GCCTCCTCCCAGTTCGTCTCAGCGCTGCTGCTGGTCGGCGCCAAATTCCGTAACGGGCTG
+GCACCTCGAACATTCCGGCCAGAGCGTCCCCAGCCTGGACCACGTTGCCATGACCGTGGC
+GGTACTGCGCAGC
+>NZ_CP018863.1_contig2
+CCCGGGAGCTTCGTCGCAGTCCGGTGGGCCTCCTCCCAGTTCGTCTCAGCGCTGCTGCTG
+GTCGGCGCCAAATTCCGTAACGGGCTGGCACCTCGAACATTCCGGCCAGAGCGTCCCCAG
+CCTGGACCACGTTGCCATGACCGTGGCGGTACTGCGCAGC
+>NZ_CP018863.1_contig3
+GTCGGCGCCAAATTCCGTAACGGGCTGGCACCTCGAACATTCCGGCCAGAGCGTCCCCAG
+GACGATCTGC

q2_moshpit/tests/test_utils.py

@@ -6,10 +6,14 @@
 # The full license is in the file LICENSE, distributed with this software.
 # ----------------------------------------------------------------------------
 import unittest
+
+import pandas as pd
 from qiime2.plugin.testing import TestPluginBase
+
+from q2_types.feature_data_mag import MAGSequencesDirFmt
 from .._utils import (
     _construct_param, _process_common_input_params,
-    _calculate_md5_from_file
+    _calculate_md5_from_file, get_feature_lengths
 )
 
 
@@ -123,6 +127,20 @@ def test_calculate_md5_from_fail(self):
         observed_hash = _calculate_md5_from_file(path_to_file)
         self.assertNotEqual(observed_hash, "a583054a9831a6e7cc56ea5cd9cac40a")
 
+    def test_get_feature_lengths(self):
+        mags = MAGSequencesDirFmt(self.get_data_path('mags-derep'), mode='r')
+        obs = get_feature_lengths(mags)
+        exp = pd.DataFrame({
+            'id': [
+                '24dee6fe-9b84-45bb-8145-de7b092533a1',
+                'ca7012fc-ba65-40c3-84f5-05aa478a7585',
+                'd65a71fa-4279-4588-b937-0747ed5d604d'
+            ],
+            'length': [66, 70, 363]
+        })
+        exp.set_index('id', inplace=True)
+        pd.testing.assert_frame_equal(obs, exp)
+
 
 if __name__ == '__main__':
     unittest.main()