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Set phred to 33 on trimmomatic task #332

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cimendes opened this issue Feb 5, 2024 · 1 comment · Fixed by #389
Closed

Set phred to 33 on trimmomatic task #332

cimendes opened this issue Feb 5, 2024 · 1 comment · Fixed by #389
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@cimendes
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cimendes commented Feb 5, 2024

🆒

Avoiding heartache and keyboard smashing on why trimmomatic is setting everything to the wrong phred encoding!

@kapsakcj
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kapsakcj commented Feb 7, 2024

agreed. Here's how I always run theiaprok_illumina_PE to avoid these trimmomatic errors. Should apply to TheiaCov workflows as well:
image

The exact string is "-phred33"

I think this could be a task-level default as it should be applied everywhere. As opposed to a workflow-level default (for example in the read_QC_trim subworkflows

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