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COVID-19_Airway

This repository contains the R scripts necessary to perform the analysis in our paper Duan, Xiaohua et al. "An Airway Organoid-Based Screen Identifies a Role for the HIF1a-Glycolysis Axis in SARS-CoV-2 Infection." Cell Reports, in press, as described in the supplementary methods and main text.

Input data

The single cell RNA-seq data were generated with the 10X Chromium and pre-processed using 10X cellranger pipeline. The raw data are available in the GEO database with accession# GSE160231.

Requirements

The following R packages are needed:

  • Seurat
  • scran
  • scater
  • future
  • dplyr
  • reshape2
  • magrittr
  • ggplot2
  • pheatmap
  • cowplot
  • RColorBrewer