Releases: sRassmann/canny3d-thresholder
Implementation of timelapse (4D) processing
Implements processing of timelapse stacks (4D).
Solves the issues with file selection on MacOS (see hansenjn/CiliaQ@5cfd95e).
Tested version
Version tested and evaluated on 3D multichannel data.
Added log file for reproducibility.
Improvements to functionality and user interface
After binarization (object detection) the tool loops over the mask and writes the original intensities in the output image. Also, the tool can extract the mask channel from a multi-channel image, also from bioformats.
Thus, this new features allow the tool to directly run on raw microscopy files and spares previous channel splitting in the CiliaQ pre-processing workflow.
Additionally, this version also includes the pattern matching as an option to detect and add files to interactive file selection dialog (Multi-File-Manager).
Initial test version
At the current state, the Plugin is experimental and meant to evaluate and improve the processing procedure. It is based purely on the IJ.run() commands and, hence requires the installation of the 3D Image Suite package.
NOTE: Right now, the plugin outputs a binarized image, thus, the intensities are overwritten and cannot be measured in downstream analysis (e.g. CiliaQ)!