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Merge pull request #17 from lmschott/patch-6
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Update capture_area_generation.md
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danilexn authored Feb 21, 2024
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Expand Up @@ -15,6 +15,10 @@ Using 200 pM library, loaded according to the KAPA qPCR value, we obtained the f
Sequence a single-end 37 cycle read, using Read1-DraI oligo as a custom primer.
Use a custom sequencing [recipe](../static/metadata_files/NovaSeq6000_S4_barcoding_seq_recipe.xml) that stops the run immediately after read 1 prior to on-instrument washes.

!!! Note
The custom recipe published in our bioarchive pre-print and linked above was provided by Illumina Technical Support. It was used in a sequencing run with the following versions: RTA v3.4.4, Flow Cell Consumable v1, Sbs Consumable v3,
NovaSeq control Software v 1.7.5 (in the pre-print) and v 1.8.1 (in an independent flow cell with no published data). Be aware that the custom recipe may change with different versions.

### Expected (data) output
Either when using your own sequencing equipment or relying on a sequencing facility, you will get access
to (most likely) already [demultiplexed](https://knowledge.illumina.com/software/general/software-general-troubleshooting-list/000005982)
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