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update docs
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andyjslee committed Nov 4, 2024
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3 changes: 2 additions & 1 deletion README.md
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Expand Up @@ -23,10 +23,11 @@ Command-line interface (CLI) for running bioinformatics workflows written in nex

Download the latest stable release [here](https://github.com/pirl-unc/nexus/releases)

Make sure to have `nextflow v24.10.0` and up:
```
conda create -n nexus python=3.10
conda activate nexus
conda install bioconda::nextflow==23.10.0
conda install bioconda::nextflow==24.10.0
pip install nexus-<version>.tar.gz --verbose
```

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Expand Up @@ -22,7 +22,7 @@ nexus run --nf-workflow long_read_rna_quantification_oarfish.nf \
--samples_tsv_file SAMPLES_TSV_FILE \
--reference_transcriptome_fasta_file REFERENCE_TRANSCRIPTOME_FASTA_FILE \
--output_dir OUTPUT_DIR \
--params_oarfish '"--seq-tech pac-bio-hifi"'
--params_oarfish '"--seq-tech pac-bio-hifi "'
```

### Usage
Expand All @@ -40,7 +40,7 @@ required arguments:
'sample_id', 'fastq_file'.
--reference_transcriptome_fasta_file : Reference transcriptome FASTA file
(default: '/datastore/lbcfs/collaborations/pirl/seqdata/references/gencode.v41.transcripts.fa').
--params_oarfish : Oarfish parameters (default: '"--seq-tech pac-bio-hifi"').
--params_oarfish : Oarfish parameters (default: '"--seq-tech pac-bio-hifi "').
Note that the parameters need to be wrapped in quotes
and a space at the end of the string is necessary.
--output_dir : Directory to which output files will be copied.
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Expand Up @@ -46,7 +46,7 @@ if (params.help) {
'sample_id', 'fastq_file'.
--reference_transcriptome_fasta_file : Reference transcriptome FASTA file
(default: '/datastore/lbcfs/collaborations/pirl/seqdata/references/gencode.v41.transcripts.fa').
--params_oarfish : Oarfish parameters (default: '"--seq-tech pac-bio-hifi"').
--params_oarfish : Oarfish parameters (default: '"--seq-tech pac-bio-hifi "').
Note that the parameters need to be wrapped in quotes
and a space at the end of the string is necessary.
--output_dir : Directory to which output files will be copied.
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Expand Up @@ -28,7 +28,7 @@ def test_long_read_rna_quantification_oarfish():
'-w', work_dir,
'--samples_tsv_file', intermediate_dir + '/samples.tsv',
'--reference_transcriptome_fasta_file', reference_transcriptome_fasta_file,
'--params_oarfish', '"--seq-tech pac-bio-hifi"',
'--params_oarfish', '"--seq-tech pac-bio-hifi "',
'--output_dir', output_dir
]
run_workflow(workflow='long_read_rna_quantification_oarfish.nf',
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