NF1 cutaneous neurofibromas (cNFs) have some similarities to non-cutaneous (plexiform) neurofibromas, but there are also differences whose molecular bases are not well characterized. There is also heterogeneity within cNFs, e.g. NF1 mutations and phenotypes. To improve our understanding of cNF biology, we will develop characterized cNF Schwann cell two-hit cultures/lines, and perform exome RNA sequence analysis in these resources as well as primary tissue (intact and isolated single cells). Heterozygous Schwann cell lines will also be developed and studied from some of the same tumors.
See the companion datasets on the NF Data Portal.
/analysis: Contains all code notebooks and rendered html notebooks.
/figures: Figures that were under consideration for inclusion in the published manuscript.
All analysis was performed in R 4.2.1. You can re-create the environment (e.g. the versions of the packages we used) with renv package and the lockfile provided in this repository.