- Fixed a bug which caused an error when using positive control sequences.
- Detection of spike sequences is now optional, and an option is also included
for positive control sequences. Both of these options are under
controls:
in the configuration file. If either type of sequences should be detected, the file containing sequences to detect should be given ascontrols: spikes:
orcontrols: positive:
respectively. Note this is a breaking change for projects that did in fact use the default synmock spikes. These are included in protaxFungi asprotaxFungi/amptk_synmock.fasta
, but must now be explicitly specified.
- Improved parsing of BOLD release datasets when used as outgroup references.
It is now possible to directly download the
BOLD_Public.DD-Mmm-YYYY.fasta.gz
to supply as the file foroutgroup_reference:sequences:
. No separate taxonomy file is required. Note that execution will be a bit faster if the FASTA file is pre-filtered to include only sequences with "COI-5P" in the header, but this is not crucial, since this is more than 80% of the sequences in recent BOLD snapshots.
Initial public release, as described in arXiv preprint.