Skip to content

Commit

Permalink
citation was updated from bioRxiv to the published paper
Browse files Browse the repository at this point in the history
  • Loading branch information
barizona committed Nov 12, 2024
1 parent 47dcc28 commit 42e397c
Show file tree
Hide file tree
Showing 8 changed files with 97 additions and 85 deletions.
2 changes: 1 addition & 1 deletion DESCRIPTION
Original file line number Diff line number Diff line change
@@ -1,6 +1,6 @@
Package: mulea
Type: Package
Title: Enrichment Analysis using Multiple Ontologies and False Discovery Rate
Title: Enrichment Analysis Using Multiple Ontologies and False Discovery Rate
Version: 1.1.0
Date: 2024-09-24
Authors@R: c(
Expand Down
2 changes: 1 addition & 1 deletion README.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -719,6 +719,6 @@ sessionInfo()

To cite package `mulea` in publications use:

Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and Eszter Ari. 2024. “mulea - an R Package for Enrichment Analysis Using Multiple Ontologies and Empirical FDR Correction.” *bioRxiv*, March. <https://doi.org/10.1101/2024.02.28.582444>.
Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and Eszter Ari. 2024. “mulea: An R Package for Enrichment Analysis Using Multiple Ontologies and Empirical False Discovery Rate.” *BMC Bioinformatics* 25 (1): 334. <https://doi.org/10.1186/s12859-024-05948-7>.

# References
137 changes: 70 additions & 67 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -388,18 +388,18 @@ ora_results %>%
filter(eFDR < 0.05)
```

| ontology_id | ontology_name | nr_common_with_tested_elements | nr_common_with_background_elements | p_value | eFDR |
|:------------|:--------------|-------------------------------:|-----------------------------------:|----------:|----------:|
| FNR | FNR | 26 | 259 | 0.0000003 | 0.0000000 |
| LexA | LexA | 14 | 53 | 0.0000000 | 0.0000000 |
| SoxS | SoxS | 7 | 37 | 0.0001615 | 0.0036667 |
| Rob | Rob | 5 | 21 | 0.0004717 | 0.0051200 |
| DnaA | DnaA | 4 | 13 | 0.0006281 | 0.0052000 |
| FadR | FadR | 5 | 20 | 0.0003692 | 0.0056000 |
| NsrR | NsrR | 8 | 64 | 0.0010478 | 0.0073714 |
| ArcA | ArcA | 12 | 148 | 0.0032001 | 0.0197500 |
| IHF | IHF | 14 | 205 | 0.0070758 | 0.0458600 |
| MarA | MarA | 5 | 37 | 0.0066068 | 0.0483111 |
| ontology_id | ontology_name | nr_common_with_tested_elements | nr_common_with_background_elements | p_value | eFDR |
|:---|:---|---:|---:|---:|---:|
| FNR | FNR | 26 | 259 | 0.0000003 | 0.0000000 |
| LexA | LexA | 14 | 53 | 0.0000000 | 0.0000000 |
| SoxS | SoxS | 7 | 37 | 0.0001615 | 0.0036667 |
| Rob | Rob | 5 | 21 | 0.0004717 | 0.0051200 |
| DnaA | DnaA | 4 | 13 | 0.0006281 | 0.0052000 |
| FadR | FadR | 5 | 20 | 0.0003692 | 0.0056000 |
| NsrR | NsrR | 8 | 64 | 0.0010478 | 0.0073714 |
| ArcA | ArcA | 12 | 148 | 0.0032001 | 0.0197500 |
| IHF | IHF | 14 | 205 | 0.0070758 | 0.0458600 |
| MarA | MarA | 5 | 37 | 0.0066068 | 0.0483111 |

### Visualising the ORA Result

Expand Down Expand Up @@ -572,27 +572,27 @@ merged_results_filtered <- merged_results %>%
-nr_common_with_background_elements)
```

| ontology_name | p_value | eFDR | BH_adjusted_p_value | Bonferroni_adjusted_p_value |
|:--------------|----------:|----------:|--------------------:|----------------------------:|
| LexA | 0.0000000 | 0.0000000 | 0.0000001 | 0.0000001 |
| FNR | 0.0000003 | 0.0000000 | 0.0000208 | 0.0000416 |
| SoxS | 0.0001615 | 0.0036667 | 0.0082880 | 0.0248641 |
| FadR | 0.0003692 | 0.0056000 | 0.0142127 | 0.0568507 |
| Rob | 0.0004717 | 0.0051200 | 0.0145296 | 0.0726479 |
| DnaA | 0.0006281 | 0.0052000 | 0.0161218 | 0.0967306 |
| NsrR | 0.0010478 | 0.0073714 | 0.0230517 | 0.1613622 |
| ArcA | 0.0032001 | 0.0197500 | 0.0616014 | 0.4928114 |
| MarA | 0.0066068 | 0.0483111 | 0.1089670 | 1.0000000 |
| IHF | 0.0070758 | 0.0458600 | 0.1089670 | 1.0000000 |
| NarL | 0.0096065 | 0.0534000 | 0.1276532 | 1.0000000 |
| NikR | 0.0099470 | 0.0615833 | 0.1276532 | 1.0000000 |
| OxyR | 0.0174505 | 0.0786923 | 0.2067212 | 1.0000000 |
| ExuR | 0.0261046 | 0.1051867 | 0.2680073 | 1.0000000 |
| UxuR | 0.0261046 | 0.1051867 | 0.2680073 | 1.0000000 |
| NrdR | 0.0328500 | 0.1232750 | 0.3161817 | 1.0000000 |
| IscR | 0.0376038 | 0.1249412 | 0.3406459 | 1.0000000 |
| Nac | 0.0419701 | 0.1487556 | 0.3590774 | 1.0000000 |
| Fis | 0.0457307 | 0.1433053 | 0.3706596 | 1.0000000 |
| ontology_name | p_value | eFDR | BH_adjusted_p_value | Bonferroni_adjusted_p_value |
|:---|---:|---:|---:|---:|
| LexA | 0.0000000 | 0.0000000 | 0.0000001 | 0.0000001 |
| FNR | 0.0000003 | 0.0000000 | 0.0000208 | 0.0000416 |
| SoxS | 0.0001615 | 0.0036667 | 0.0082880 | 0.0248641 |
| FadR | 0.0003692 | 0.0056000 | 0.0142127 | 0.0568507 |
| Rob | 0.0004717 | 0.0051200 | 0.0145296 | 0.0726479 |
| DnaA | 0.0006281 | 0.0052000 | 0.0161218 | 0.0967306 |
| NsrR | 0.0010478 | 0.0073714 | 0.0230517 | 0.1613622 |
| ArcA | 0.0032001 | 0.0197500 | 0.0616014 | 0.4928114 |
| MarA | 0.0066068 | 0.0483111 | 0.1089670 | 1.0000000 |
| IHF | 0.0070758 | 0.0458600 | 0.1089670 | 1.0000000 |
| NarL | 0.0096065 | 0.0534000 | 0.1276532 | 1.0000000 |
| NikR | 0.0099470 | 0.0615833 | 0.1276532 | 1.0000000 |
| OxyR | 0.0174505 | 0.0786923 | 0.2067212 | 1.0000000 |
| ExuR | 0.0261046 | 0.1051867 | 0.2680073 | 1.0000000 |
| UxuR | 0.0261046 | 0.1051867 | 0.2680073 | 1.0000000 |
| NrdR | 0.0328500 | 0.1232750 | 0.3161817 | 1.0000000 |
| IscR | 0.0376038 | 0.1249412 | 0.3406459 | 1.0000000 |
| Nac | 0.0419701 | 0.1487556 | 0.3590774 | 1.0000000 |
| Fis | 0.0457307 | 0.1433053 | 0.3706596 | 1.0000000 |

A comparison of the significant results revealed that conventional
*p*-value corrections (Benjamini-Hochberg and Bonferroni) tend to be
Expand Down Expand Up @@ -690,7 +690,7 @@ gsea_results %>%
filter(adjusted_p_value < 0.05) %>%
# the number of such rows
nrow()
#> [1] 9
#> [1] 10
```

Inspect the significant results:
Expand All @@ -703,17 +703,18 @@ gsea_results %>%
filter(adjusted_p_value < 0.05)
```

| ontology_id | ontology_name | nr_common_with_tested_elements | p_value | adjusted_p_value |
|:------------|:--------------|-------------------------------:|----------:|-----------------:|
| LexA | LexA | 53 | 0.0000001 | 0.0000089 |
| FNR | FNR | 259 | 0.0000379 | 0.0028982 |
| GlaR | GlaR | 3 | 0.0002188 | 0.0111583 |
| ArcA | ArcA | 148 | 0.0003258 | 0.0117944 |
| DnaA | DnaA | 13 | 0.0004397 | 0.0117944 |
| ModE | ModE | 45 | 0.0004625 | 0.0117944 |
| SoxS | SoxS | 37 | 0.0007464 | 0.0163152 |
| PspF | PspF | 7 | 0.0016107 | 0.0308049 |
| PaaX | PaaX | 14 | 0.0019054 | 0.0323922 |
| ontology_id | ontology_name | nr_common_with_tested_elements | p_value | adjusted_p_value |
|:---|:---|---:|---:|---:|
| LexA | LexA | 53 | 0.0000000 | 0.0000047 |
| FNR | FNR | 259 | 0.0000660 | 0.0050484 |
| ArcA | ArcA | 148 | 0.0003076 | 0.0079598 |
| GlaR | GlaR | 3 | 0.0002188 | 0.0079598 |
| ModE | ModE | 45 | 0.0003122 | 0.0079598 |
| SoxS | SoxS | 37 | 0.0002848 | 0.0079598 |
| DnaA | DnaA | 13 | 0.0010217 | 0.0223306 |
| PaaX | PaaX | 14 | 0.0017028 | 0.0325652 |
| PspF | PspF | 7 | 0.0023494 | 0.0399397 |
| FadR | FadR | 20 | 0.0028304 | 0.0433046 |

### Visualising the GSEA Results

Expand Down Expand Up @@ -781,11 +782,11 @@ geo2r_result_tab %>%
head(3)
```

| ID | adj.P.Val | P.Value | t | B | logFC | Gene.symbol | Gene.title |
|:-------------|----------:|--------:|-----:|--------:|------:|:-----------------------------|:----------------------------------------------------------------------------------------------------------------------|
| 1765336_s_at | 0.0186 | 2.4e-06 | 21.5 | 4.95769 | 3.70 | gnsB | Qin prophage; multicopy suppressor of secG(Cs) and fabA6(Ts) |
| 1760422_s_at | 0.0186 | 3.8e-06 | 19.6 | 4.68510 | 3.14 | NA | NA |
| 1764904_s_at | 0.0186 | 5.7e-06 | 18.2 | 4.43751 | 2.54 | sulA///sulA///sulA///ECs1042 | SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor |
| ID | adj.P.Val | P.Value | t | B | logFC | Gene.symbol | Gene.title |
|:---|---:|---:|---:|---:|---:|:---|:---|
| 1765336_s_at | 0.0186 | 2.4e-06 | 21.5 | 4.95769 | 3.70 | gnsB | Qin prophage; multicopy suppressor of secG(Cs) and fabA6(Ts) |
| 1760422_s_at | 0.0186 | 3.8e-06 | 19.6 | 4.68510 | 3.14 | NA | NA |
| 1764904_s_at | 0.0186 | 5.7e-06 | 18.2 | 4.43751 | 2.54 | sulA///sulA///sulA///ECs1042 | SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor |

### **Data Preparation:**

Expand Down Expand Up @@ -824,11 +825,11 @@ geo2r_result_tab %>%
head(3)
```

| ID | adj.P.Val | P.Value | t | B | logFC | Gene.symbol | Gene.title |
|:-------------|----------:|---------:|-----:|--------:|------:|:------------|:------------------------------------------------------------------------------------------------------------------------------------------|
| 1765336_s_at | 0.0186 | 2.40e-06 | 21.5 | 4.95769 | 3.70 | gnsB | Qin prophage; multicopy suppressor of secG(Cs) and fabA6(Ts) |
| 1764904_s_at | 0.0186 | 5.70e-06 | 18.2 | 4.43751 | 2.54 | sulA | SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor |
| 1761763_s_at | 0.0186 | 1.54e-05 | 15.0 | 3.73568 | 2.16 | recN | recombination and repair protein///recombination and repair protein///recombination and repair protein///recombination and repair protein |
| ID | adj.P.Val | P.Value | t | B | logFC | Gene.symbol | Gene.title |
|:---|---:|---:|---:|---:|---:|:---|:---|
| 1765336_s_at | 0.0186 | 2.40e-06 | 21.5 | 4.95769 | 3.70 | gnsB | Qin prophage; multicopy suppressor of secG(Cs) and fabA6(Ts) |
| 1764904_s_at | 0.0186 | 5.70e-06 | 18.2 | 4.43751 | 2.54 | sulA | SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor///SOS cell division inhibitor |
| 1761763_s_at | 0.0186 | 1.54e-05 | 15.0 | 3.73568 | 2.16 | recN | recombination and repair protein///recombination and repair protein///recombination and repair protein///recombination and repair protein |

Following these formatting steps, the data frame is primed for further
analysis.
Expand Down Expand Up @@ -942,7 +943,7 @@ ordered_set %>%

``` r
sessionInfo()
#> R version 4.4.1 (2024-06-14)
#> R version 4.4.2 (2024-10-31)
#> Platform: x86_64-pc-linux-gnu
#> Running under: Ubuntu 22.04.5 LTS
#>
Expand Down Expand Up @@ -972,18 +973,18 @@ sessionInfo()
#> loaded via a namespace (and not attached):
#> [1] fastmatch_1.1-4 gtable_0.3.5 xfun_0.47
#> [4] ggrepel_0.9.6 lattice_0.22-6 tzdb_0.4.0
#> [7] vctrs_0.6.5 tools_4.4.1 generics_0.1.3
#> [10] curl_5.2.2 parallel_4.4.1 fansi_1.0.6
#> [7] vctrs_0.6.5 tools_4.4.2 generics_0.1.3
#> [10] curl_5.2.2 parallel_4.4.2 fansi_1.0.6
#> [13] highr_0.11 pkgconfig_2.0.3 Matrix_1.7-0
#> [16] data.table_1.16.0 lifecycle_1.0.4 compiler_4.4.1
#> [16] data.table_1.16.0 lifecycle_1.0.4 compiler_4.4.2
#> [19] farver_2.1.2 munsell_0.5.1 ggforce_0.4.2
#> [22] fgsea_1.30.0 graphlayouts_1.1.1 codetools_0.2-20
#> [22] fgsea_1.30.0 graphlayouts_1.2.0 codetools_0.2-20
#> [25] htmltools_0.5.8.1 yaml_2.3.10 crayon_1.5.3
#> [28] pillar_1.9.0 MASS_7.3-61 BiocParallel_1.38.0
#> [31] cachem_1.1.0 viridis_0.6.5 tidyselect_1.2.1
#> [34] digest_0.6.37 stringi_1.8.4 labeling_0.4.3
#> [37] cowplot_1.1.3 polyclip_1.10-7 fastmap_1.2.0
#> [40] grid_4.4.1 colorspace_2.1-1 cli_3.6.3
#> [40] grid_4.4.2 colorspace_2.1-1 cli_3.6.3
#> [43] magrittr_2.0.3 ggraph_2.2.1 tidygraph_1.3.1
#> [46] utf8_1.2.4 withr_3.0.1 scales_1.3.0
#> [49] bit64_4.0.5 timechange_0.3.0 rmarkdown_2.28
Expand All @@ -1001,9 +1002,10 @@ To cite package `mulea` in publications use:

Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin
Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and
Eszter Ari. 2024. “mulea - an R Package for Enrichment Analysis Using
Multiple Ontologies and Empirical FDR Correction.” *bioRxiv*, March.
<https://doi.org/10.1101/2024.02.28.582444>.
Eszter Ari. 2024. “mulea: An R Package for Enrichment Analysis Using
Multiple Ontologies and Empirical False Discovery Rate.” *BMC
Bioinformatics* 25 (1): 334.
<https://doi.org/10.1186/s12859-024-05948-7>.

# References

Expand Down Expand Up @@ -1052,9 +1054,10 @@ National Academy of Sciences* 102 (43): 15545–50.

Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin
Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and
Eszter Ari. 2024. “Mulea - an R Package for Enrichment Analysis Using
Multiple Ontologies and Empirical FDR Correction.” *bioRxiv*, March.
<https://doi.org/10.1101/2024.02.28.582444>.
Eszter Ari. 2024. “<span class="nocase">mulea</span>: An R Package for
Enrichment Analysis Using Multiple Ontologies and Empirical False
Discovery Rate.” *BMC Bioinformatics* 25 (1): 334.
<https://doi.org/10.1186/s12859-024-05948-7>.

</div>

Expand Down
19 changes: 12 additions & 7 deletions inst/CITATION
Original file line number Diff line number Diff line change
@@ -1,4 +1,4 @@
citHeader("To cite mulea in publications use:")
citHeader("Please cite the following paper when using the mulea package:")

bibentry(bibtype = "Article",
author = c(
Expand All @@ -14,14 +14,19 @@ bibentry(bibtype = "Article",
person("Eszter", "Ari")
),
title = "mulea - an R package for enrichment analysis using multiple
ontologies and empirical FDR correction.",
ontologies and empirical false discovery rate.",
journal = "BMC Bioinformatics",
year = "2024",
publisher = "Cold Spring Harbor Laboratory",
URL = "https://doi.org/10.1101/2024.02.28.582444",
journal = "bioRxiv",
volume = "25",
number = "1",
pages = "334",
URL = "https://doi.org/10.1186/s12859-024-05948-7",

textVersion = paste("C. Turek, M. Olbei, T. Stirling, G. Fekete, E. Tasnadi,
L. Gul, B. Bohar, B. Papp, W. Jurkowski, E. Ari:",
"mulea - an R package for enrichment analysis using multiple ontologies
and empirical FDR correction.",
"bioRxiv (2024), doi:10.1101/2024.02.28.582444v1")
and empirical false discovery rate.",
"BMC Bioinformatics, 25(1): 334 (2024), doi:10.1186/s12859-024-05948-7")
)

citFooter("Thank you for using our package!")
Binary file modified man/figures/README-network_plot_gsea-1.png
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
Binary file modified man/figures/README-network_plot_ora-1.png
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
18 changes: 11 additions & 7 deletions references.bib
Original file line number Diff line number Diff line change
Expand Up @@ -34,17 +34,21 @@ @article{kuleshov2016
}

@article{turek,
title = {mulea - an {R} package for enrichment analysis using multiple ontologies and empirical {FDR} correction},
url = {https://www.biorxiv.org/content/10.1101/2024.02.28.582444v1},
doi = {10.1101/2024.02.28.582444},
language = {en},
title = {{mulea}: {An} {R} package for enrichment analysis using multiple ontologies and empirical false discovery rate},
author = {Turek, Cezary and {Ölbei}, {Márton} and Stirling, {Tamás} and Fekete, Gergely and {Tasnádi}, Ervin and Gul, Leila and {Bohár}, {Balázs} and Papp, {Balázs} and Jurkowski, Wiktor and Ari, Eszter},
date = {2024-03-01},
journal = {bioRxiv}
year = {2024},
month = {10},
date = {2024-10-18},
journal = {BMC Bioinformatics},
pages = {334},
volume = {25},
number = {1},
doi = {10.1186/s12859-024-05948-7},
url = {https://doi.org/10.1186/s12859-024-05948-7}
}

@article{méhi2014,
title = {Perturbation of Iron Homeostasis Promotes the Evolution of Antibiotic Resistance},
title = {Perturbation of iron homeostasis promotes the evolution of antibiotic resistance},
author = {{Méhi}, Orsolya and Bogos, {Balázs} and {Csörg{{\H o}}}, {Bálint} and {Pál}, Ferenc and Nyerges, {Ákos} and Papp, {Balázs} and {Pál}, Csaba},
year = {2014},
month = {10},
Expand Down
4 changes: 2 additions & 2 deletions vignettes/mulea.Rmd
Original file line number Diff line number Diff line change
Expand Up @@ -728,7 +728,7 @@ sessionInfo()

To cite package `mulea` in publications use:

Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and Eszter Ari. 2024. “mulea - an R Package for Enrichment Analysis Using Multiple Ontologies and Empirical FDR Correction.” *bioRxiv*, March. <https://doi.org/10.1101/2024.02.28.582444>.
Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and Eszter Ari. 2024. “mulea: An R Package for Enrichment Analysis Using Multiple Ontologies and Empirical False Discovery Rate.” *BMC Bioinformatics* 25 (1): 334. <https://doi.org/10.1186/s12859-024-05948-7>.

# References

Expand All @@ -740,4 +740,4 @@ Méhi, Orsolya, Balázs Bogos, Bálint Csörgő, Ferenc Pál, Ákos Nyerges, Bal

Subramanian, Aravind, Pablo Tamayo, Vamsi K. Mootha, Sayan Mukherjee, Benjamin L. Ebert, Michael A. Gillette, Amanda Paulovich, et al. 2005. “Gene Set Enrichment Analysis: A Knowledge-Based Approach for Interpreting Genome-Wide Expression Profiles.” *Proceedings of the National Academy of Sciences* 102 (43): 15545–50. <https://doi.org/10.1073/pnas.0506580102>.

Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and Eszter Ari. 2024. “Mulea - an R Package for Enrichment Analysis Using Multiple Ontologies and Empirical FDR Correction.” *bioRxiv*, March. <https://doi.org/10.1101/2024.02.28.582444>.
Turek, Cezary, Márton Ölbei, Tamás Stirling, Gergely Fekete, Ervin Tasnádi, Leila Gul, Balázs Bohár, Balázs Papp, Wiktor Jurkowski, and Eszter Ari. 2024. “mulea: An R Package for Enrichment Analysis Using Multiple Ontologies and Empirical False Discovery Rate.” *BMC Bioinformatics* 25 (1): 334. <https://doi.org/10.1186/s12859-024-05948-7>.

0 comments on commit 42e397c

Please sign in to comment.