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Merge pull request #42 from Danderson123/dev
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Danderson123 authored Jan 24, 2025
2 parents a4d8acb + 839f58b commit dfab027
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4 changes: 2 additions & 2 deletions README.md
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Expand Up @@ -73,7 +73,7 @@ python3 amira/__main__.py --pandoraJSON <PATH TO GENE CALL JSON> --gene-position

Some example JSON data can be downloaded from [here](https://drive.google.com/drive/folders/1mQ8JmzVhFiNkgRy5_1iFQrqV2TLNnlQ4). Amira can then be run using this command:
```
python3 amira/__main__.py --pandoraJSON test_data/gene_calls_with_gene_filtering.json --gene-positions test_data/gene_positions_with_gene_filtering.json --pandoraConsensus test_data/pandora.consensus.fq.gz --readfile test_data/SRR23044220_1.fastq.gz --output amira_output --gene-path AMR_alleles_unified.fa --phenotypes AMR_calls.json --racon-path <PATH TO RACON BINARY> --minimap2-path <PATH TO MINIMAP2 BINARY> --debug --cores <CPUS> --sample-reads --filter-contaminants
python3 amira/__main__.py --pandoraJSON test_data/gene_calls_with_gene_filtering.json --gene-positions test_data/gene_positions_with_gene_filtering.json --pandoraConsensus test_data/pandora.consensus.fq.gz --readfile test_data/SRR23044220_1.fastq.gz --output amira_output --gene-path E.coli/AMR_alleles_unified.fa --annotation E.coli/AMR_calls.json --racon-path <PATH TO RACON BINARY> --minimap2-path <PATH TO MINIMAP2 BINARY> --debug --cores <CPUS> --sample-reads --filter-contaminants
```

### Running with Pandora
Expand All @@ -83,7 +83,7 @@ pandora map -t <THREADS> --min-gene-coverage-proportion 0.5 --max-covg 10000 -o
```
Amira can then be run directly on the output of Pandora using this command:
```bash
python3 amira/__main__.py --pandoraSam pandora_map_output/*.sam --pandoraConsensus pandora_map_output/pandora.consensus.fq.gz --readfile <PATH TO READ FASTQ> --output amira_output --gene-path AMR_alleles_unified.fa --minimum-length-proportion 0.5 --maximum-length-proportion 1.5 --cores <CPUS> --phenotypes AMR_calls.json --filter-contaminants --sample-reads
python3 amira/__main__.py --pandoraSam pandora_map_output/*.sam --pandoraConsensus pandora_map_output/pandora.consensus.fq.gz --readfile <PATH TO READ FASTQ> --output amira_output --gene-path E.coli/AMR_alleles_unified.fa --minimum-length-proportion 0.5 --maximum-length-proportion 1.5 --cores <CPUS> --annotation E.coli/AMR_calls.json --filter-contaminants --sample-reads
```

### Additional options
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