diff --git a/spyder/plugins/variableexplorer/widgets/collectionsdelegate.py b/spyder/plugins/variableexplorer/widgets/collectionsdelegate.py
index 218eaffc197..4b875371514 100644
--- a/spyder/plugins/variableexplorer/widgets/collectionsdelegate.py
+++ b/spyder/plugins/variableexplorer/widgets/collectionsdelegate.py
@@ -19,6 +19,8 @@
 from qtpy.QtCore import (
     QDateTime,
     QEvent,
+    QItemSelection,
+    QItemSelectionModel,
     QModelIndex,
     QRect,
     QSize,
@@ -525,7 +527,11 @@ def paint(self, painter, option, index):
                 x, y, SELECT_ROW_BUTTON_SIZE, SELECT_ROW_BUTTON_SIZE
             )
             button.text = ""
-            button.icon = ima.icon("select_row")
+            button.icon = (
+                ima.icon("select_row")
+                if index.row() not in self.parent().selected_rows()
+                else ima.icon("deselect_row")
+            )
             button.iconSize = QSize(20, 20)
             button.state = QStyle.State_Enabled
             QApplication.style().drawControl(
@@ -552,9 +558,19 @@ def editorEvent(self, event, model, option, index):
             x = rect.left() + rect.width() - SELECT_ROW_BUTTON_SIZE
             y = rect.top()
 
-            # Select row when clicking on the button
+            # Select/deselect row when clicking on the button
             if click_x > x and (y < click_y < (y + SELECT_ROW_BUTTON_SIZE)):
-                self.parent().selectRow(index.row())
+                row = index.row()
+                if row in self.parent().selected_rows():
+                    # Deselect row if selected
+                    index_left = index.sibling(row, 0)
+                    index_right = index.sibling(row, 3)
+                    selection = QItemSelection(index_left, index_right)
+                    self.parent().selectionModel().select(
+                        selection, QItemSelectionModel.Deselect
+                    )
+                else:
+                    self.parent().selectRow(row)
             else:
                 super().editorEvent(event, model, option, index)
         else:
diff --git a/spyder/utils/icon_manager.py b/spyder/utils/icon_manager.py
index 16125a476b7..1ce13d1216c 100644
--- a/spyder/utils/icon_manager.py
+++ b/spyder/utils/icon_manager.py
@@ -215,6 +215,7 @@ def __init__(self):
             'move':                    [('mdi.file-move',), {'color': self.MAIN_FG_COLOR}],
             'edit_add':                [('mdi.plus-box',), {'color': self.MAIN_FG_COLOR}],
             'select_row':              [('mdi.plus-box-outline',), {'color': self.MAIN_FG_COLOR}],
+            'deselect_row':            [('mdi.minus-box-outline',), {'color': self.MAIN_FG_COLOR}],
             'duplicate_row':           [('ph.rows',), {'color': self.MAIN_FG_COLOR}],
             'duplicate_column':        [('ph.columns',), {'color': self.MAIN_FG_COLOR}],
             'collapse_column':         [('mdi.arrow-collapse-horizontal',), {'color': self.MAIN_FG_COLOR}],
diff --git a/spyder/widgets/collectionseditor.py b/spyder/widgets/collectionseditor.py
index c1510bc0b6a..41e6ee5522a 100644
--- a/spyder/widgets/collectionseditor.py
+++ b/spyder/widgets/collectionseditor.py
@@ -483,10 +483,13 @@ def data(self, index, role=Qt.DisplayRole):
                 display = value
         if role == Qt.ToolTipRole:
             if self.parent().over_select_row_button:
-                tooltip = _(
-                    "Click to select this row. Maintain pressed Ctrl (Cmd on "
-                    "macOS) for multiple rows"
-                )
+                if index.row() in self.parent().selected_rows():
+                    tooltip = _("Click to deselect this row")
+                else:
+                    tooltip = _(
+                        "Click to select this row. Maintain pressed Ctrl (Cmd "
+                        "on macOS) for multiple rows"
+                    )
                 return '\n'.join(textwrap.wrap(tooltip, 50))
             return display
         if role == Qt.UserRole:
@@ -1500,6 +1503,12 @@ def paste(self):
             QMessageBox.warning(self, _( "Empty clipboard"),
                                 _("Nothing to be imported from clipboard."))
 
+    def selected_rows(self):
+        """Get the rows currently selected."""
+        return [
+            index.row() for index in self.selectionModel().selectedRows()
+        ]
+
 
 class CollectionsEditorTableView(BaseTableView):
     """CollectionsEditor table view"""