diff --git a/Doc/library/sys.rst b/Doc/library/sys.rst
index b0e40a4ea06946..ab16c36201e6db 100644
--- a/Doc/library/sys.rst
+++ b/Doc/library/sys.rst
@@ -1727,6 +1727,8 @@ always available.
    Activate the stack profiler trampoline *backend*.
    The only supported backend is ``"perf"``.
 
+   Stack trampolines cannot be activated if the JIT is active.
+
    .. availability:: Linux.
 
    .. versionadded:: 3.12
diff --git a/Misc/NEWS.d/next/Core_and_Builtins/2024-10-01-17-31-32.gh-issue-124855.sdsv_H.rst b/Misc/NEWS.d/next/Core_and_Builtins/2024-10-01-17-31-32.gh-issue-124855.sdsv_H.rst
new file mode 100644
index 00000000000000..b65a5e6ac11c76
--- /dev/null
+++ b/Misc/NEWS.d/next/Core_and_Builtins/2024-10-01-17-31-32.gh-issue-124855.sdsv_H.rst
@@ -0,0 +1,2 @@
+Don't allow the JIT and perf support to be active at the same time. Patch by
+Pablo Galindo
diff --git a/Python/pylifecycle.c b/Python/pylifecycle.c
index 8aebbe5c405ffe..9be1a5dac2fc6e 100644
--- a/Python/pylifecycle.c
+++ b/Python/pylifecycle.c
@@ -1300,14 +1300,21 @@ init_interp_main(PyThreadState *tstate)
             enabled = *env != '0';
         }
         if (enabled) {
-            PyObject *opt = _PyOptimizer_NewUOpOptimizer();
-            if (opt == NULL) {
-                return _PyStatus_ERR("can't initialize optimizer");
-            }
-            if (_Py_SetTier2Optimizer((_PyOptimizerObject *)opt)) {
-                return _PyStatus_ERR("can't install optimizer");
+            if (config->perf_profiling > 0) {
+                (void)PyErr_WarnEx(
+                    PyExc_RuntimeWarning,
+                    "JIT deactivated as perf profiling support is active",
+                    0);
+            } else {
+                PyObject *opt = _PyOptimizer_NewUOpOptimizer();
+                if (opt == NULL) {
+                    return _PyStatus_ERR("can't initialize optimizer");
+                }
+                if (_Py_SetTier2Optimizer((_PyOptimizerObject *)opt)) {
+                    return _PyStatus_ERR("can't install optimizer");
+                }
+                Py_DECREF(opt);
             }
-            Py_DECREF(opt);
         }
     }
 #endif
diff --git a/Python/sysmodule.c b/Python/sysmodule.c
index ac343a8048e008..5bdfca549e08d1 100644
--- a/Python/sysmodule.c
+++ b/Python/sysmodule.c
@@ -2287,6 +2287,14 @@ sys_activate_stack_trampoline_impl(PyObject *module, const char *backend)
 /*[clinic end generated code: output=5783cdeb51874b43 input=a12df928758a82b4]*/
 {
 #ifdef PY_HAVE_PERF_TRAMPOLINE
+#ifdef _Py_JIT
+    _PyOptimizerObject* optimizer = _Py_GetOptimizer();
+    if (optimizer != NULL) {
+        PyErr_SetString(PyExc_ValueError, "Cannot activate the perf trampoline if the JIT is active");
+        return NULL;
+    }
+#endif
+
     if (strcmp(backend, "perf") == 0) {
         _PyPerf_Callbacks cur_cb;
         _PyPerfTrampoline_GetCallbacks(&cur_cb);