diff --git a/docs/changelog.rst b/docs/changelog.rst
index 990ec51c..5e31413a 100644
--- a/docs/changelog.rst
+++ b/docs/changelog.rst
@@ -9,6 +9,10 @@ Compatibility
 
 * Added official support for Python 3.13.
 
+* Added ``using`` argument to :fixture:`django_assert_num_queries` and
+  :fixture:`django_assert_max_num_queries` to easily specify the database
+  alias to use.
+
 Bugfixes
 ^^^^^^^^
 
diff --git a/docs/helpers.rst b/docs/helpers.rst
index e5e4ed36..c9e189dd 100644
--- a/docs/helpers.rst
+++ b/docs/helpers.rst
@@ -423,11 +423,12 @@ Example
 ``django_assert_num_queries``
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 
-.. py:function:: django_assert_num_queries(num, connection=None, info=None)
+.. py:function:: django_assert_num_queries(num, connection=None, info=None, *, using=None)
 
   :param num: expected number of queries
-  :param connection: optional non-default DB connection
+  :param connection: optional database connection
   :param str info: optional info message to display on failure
+  :param str using: optional database alias
 
 This fixture allows to check for an expected number of DB queries.
 
@@ -462,11 +463,12 @@ If you use type annotations, you can annotate the fixture like this::
 ``django_assert_max_num_queries``
 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
 
-.. py:function:: django_assert_max_num_queries(num, connection=None, info=None)
+.. py:function:: django_assert_max_num_queries(num, connection=None, info=None, *, using=None)
 
   :param num: expected maximum number of queries
-  :param connection: optional non-default DB connection
+  :param connection: optional database connection
   :param str info: optional info message to display on failure
+  :param str using: optional database alias
 
 This fixture allows to check for an expected maximum number of DB queries.
 
diff --git a/pytest_django/fixtures.py b/pytest_django/fixtures.py
index 1daab118..39c84b60 100644
--- a/pytest_django/fixtures.py
+++ b/pytest_django/fixtures.py
@@ -606,6 +606,8 @@ def __call__(
         num: int,
         connection: Any | None = ...,
         info: str | None = ...,
+        *,
+        using: str | None = ...,
     ) -> django.test.utils.CaptureQueriesContext:
         pass  # pragma: no cover
 
@@ -617,13 +619,21 @@ def _assert_num_queries(
     exact: bool = True,
     connection: Any | None = None,
     info: str | None = None,
+    *,
+    using: str | None = None,
 ) -> Generator[django.test.utils.CaptureQueriesContext, None, None]:
+    from django.db import connection as default_conn, connections
     from django.test.utils import CaptureQueriesContext
 
-    if connection is None:
-        from django.db import connection as conn
-    else:
+    if connection and using:
+        raise ValueError('The "connection" and "using" parameter cannot be used together')
+
+    if connection is not None:
         conn = connection
+    elif using is not None:
+        conn = connections[using]
+    else:
+        conn = default_conn
 
     verbose = config.getoption("verbose") > 0
     with CaptureQueriesContext(conn) as context:
diff --git a/tests/test_fixtures.py b/tests/test_fixtures.py
index dd695136..39c6666f 100644
--- a/tests/test_fixtures.py
+++ b/tests/test_fixtures.py
@@ -15,6 +15,7 @@
 from django.core import mail
 from django.db import connection, transaction
 from django.test import AsyncClient, AsyncRequestFactory, Client, RequestFactory
+from django.utils.connection import ConnectionDoesNotExist
 from django.utils.encoding import force_str
 
 from .helpers import DjangoPytester
@@ -206,6 +207,28 @@ def test_django_assert_num_queries_db_connection(
             pass
 
 
+@pytest.mark.django_db
+def test_django_assert_num_queries_db_using(
+    django_assert_num_queries: DjangoAssertNumQueries,
+) -> None:
+    from django.db import connection
+
+    with django_assert_num_queries(1, using="default"):
+        Item.objects.create(name="foo")
+
+    error_message = 'The "connection" and "using" parameter cannot be used together'
+    with pytest.raises(ValueError, match=error_message):
+        with django_assert_num_queries(1, connection=connection, using="default"):
+            Item.objects.create(name="foo")
+
+    with django_assert_num_queries(1, using=None):
+        Item.objects.create(name="foo")
+
+    with pytest.raises(ConnectionDoesNotExist):
+        with django_assert_num_queries(1, using="bad_db_name"):
+            pass
+
+
 @pytest.mark.django_db
 def test_django_assert_num_queries_output_info(django_pytester: DjangoPytester) -> None:
     django_pytester.create_test_module(