diff --git a/tests/integration/conftest.py b/tests/integration/conftest.py
index 3e13928920..a2f1c2e7ed 100644
--- a/tests/integration/conftest.py
+++ b/tests/integration/conftest.py
@@ -115,6 +115,7 @@ def isolate(pathlib_tmpdir):
     os.environ["WORKON_HOME"] = fs_str(os.path.join(home_dir, ".virtualenvs"))
     # Ignore PIPENV_ACTIVE so that it works as under a bare environment.
     os.environ.pop("PIPENV_ACTIVE", None)
+    os.environ.pop("VIRTUAL_ENV", None)
     global WE_HAVE_GITHUB_SSH_KEYS
     WE_HAVE_GITHUB_SSH_KEYS = check_github_ssh()