diff --git a/doc/source/whatsnew/v2.0.0.rst b/doc/source/whatsnew/v2.0.0.rst
index 6239ddf9442e7..736beffe0418e 100644
--- a/doc/source/whatsnew/v2.0.0.rst
+++ b/doc/source/whatsnew/v2.0.0.rst
@@ -194,6 +194,7 @@ Removal of prior version deprecations/changes
 - Remove :meth:`DataFrameGroupBy.pad` and :meth:`DataFrameGroupBy.backfill` (:issue:`45076`)
 - Remove ``numpy`` argument from :func:`read_json` (:issue:`30636`)
 - Removed the ``center`` keyword in :meth:`DataFrame.expanding` (:issue:`20647`)
+- Removed the ``truediv`` keyword from :func:`eval` (:issue:`29812`)
 - Removed the ``pandas.datetime`` submodule (:issue:`30489`)
 - Removed the ``pandas.np`` submodule (:issue:`30296`)
 - Removed ``pandas.SparseArray`` in favor of :class:`arrays.SparseArray` (:issue:`30642`)
diff --git a/pandas/core/computation/eval.py b/pandas/core/computation/eval.py
index baf8c837d7f78..bc8c37b9273ce 100644
--- a/pandas/core/computation/eval.py
+++ b/pandas/core/computation/eval.py
@@ -7,8 +7,6 @@
 from typing import TYPE_CHECKING
 import warnings
 
-from pandas._libs.lib import no_default
-from pandas.util._exceptions import find_stack_level
 from pandas.util._validators import validate_bool_kwarg
 
 from pandas.core.computation.engines import ENGINES
@@ -171,7 +169,6 @@ def eval(
     expr: str | BinOp,  # we leave BinOp out of the docstr bc it isn't for users
     parser: str = "pandas",
     engine: str | None = None,
-    truediv=no_default,
     local_dict=None,
     global_dict=None,
     resolvers=(),
@@ -217,12 +214,6 @@ def eval(
           level python. This engine is generally not that useful.
 
         More backends may be available in the future.
-
-    truediv : bool, optional
-        Whether to use true division, like in Python >= 3.
-
-        .. deprecated:: 1.0.0
-
     local_dict : dict or None, optional
         A dictionary of local variables, taken from locals() by default.
     global_dict : dict or None, optional
@@ -305,16 +296,6 @@ def eval(
     """
     inplace = validate_bool_kwarg(inplace, "inplace")
 
-    if truediv is not no_default:
-        warnings.warn(
-            (
-                "The `truediv` parameter in pd.eval is deprecated and "
-                "will be removed in a future version."
-            ),
-            FutureWarning,
-            stacklevel=find_stack_level(),
-        )
-
     exprs: list[str | BinOp]
     if isinstance(expr, str):
         _check_expression(expr)
diff --git a/pandas/tests/computation/test_eval.py b/pandas/tests/computation/test_eval.py
index 6548422c5d2b7..7fce4e9d9c38e 100644
--- a/pandas/tests/computation/test_eval.py
+++ b/pandas/tests/computation/test_eval.py
@@ -1105,40 +1105,6 @@ def test_single_variable(self):
         df2 = self.eval("df", local_dict={"df": df})
         tm.assert_frame_equal(df, df2)
 
-    def test_truediv(self):
-        s = np.array([1])  # noqa:F841
-        ex = "s / 1"
-
-        # FutureWarning: The `truediv` parameter in pd.eval is deprecated and will be
-        # removed in a future version.
-        with tm.assert_produces_warning(FutureWarning):
-            res = self.eval(ex, truediv=False)
-        tm.assert_numpy_array_equal(res, np.array([1.0]))
-
-        with tm.assert_produces_warning(FutureWarning):
-            res = self.eval(ex, truediv=True)
-        tm.assert_numpy_array_equal(res, np.array([1.0]))
-
-        with tm.assert_produces_warning(FutureWarning):
-            res = self.eval("1 / 2", truediv=True)
-        expec = 0.5
-        assert res == expec
-
-        with tm.assert_produces_warning(FutureWarning):
-            res = self.eval("1 / 2", truediv=False)
-        expec = 0.5
-        assert res == expec
-
-        with tm.assert_produces_warning(FutureWarning):
-            res = self.eval("s / 2", truediv=False)
-        expec = 0.5
-        assert res == expec
-
-        with tm.assert_produces_warning(FutureWarning):
-            res = self.eval("s / 2", truediv=True)
-        expec = 0.5
-        assert res == expec
-
     def test_failing_subscript_with_name_error(self):
         df = DataFrame(np.random.randn(5, 3))  # noqa:F841
         with pytest.raises(NameError, match="name 'x' is not defined"):
@@ -1859,23 +1825,6 @@ def test_inf(engine, parser):
     assert result == expected
 
 
-def test_truediv_deprecated(engine, parser):
-    # GH#29182
-    match = "The `truediv` parameter in pd.eval is deprecated"
-
-    with tm.assert_produces_warning(FutureWarning) as m:
-        pd.eval("1+1", engine=engine, parser=parser, truediv=True)
-
-    assert len(m) == 1
-    assert match in str(m[0].message)
-
-    with tm.assert_produces_warning(FutureWarning) as m:
-        pd.eval("1+1", engine=engine, parser=parser, truediv=False)
-
-    assert len(m) == 1
-    assert match in str(m[0].message)
-
-
 @pytest.mark.parametrize("column", ["Temp(°C)", "Capacitance(μF)"])
 def test_query_token(engine, column):
     # See: https://github.com/pandas-dev/pandas/pull/42826