diff --git a/tests/e2e/creation_test.go b/tests/e2e/creation_test.go
index 2a2abc3cd2b..892414014e6 100644
--- a/tests/e2e/creation_test.go
+++ b/tests/e2e/creation_test.go
@@ -6,12 +6,14 @@ import (
 	"fmt"
 	"log"
 	"reflect"
+	"strings"
 	"testing"
 	"time"
 
 	operatorv1 "github.com/openshift/api/operator/v1"
 	"github.com/stretchr/testify/require"
 	autoscalingv1 "k8s.io/api/autoscaling/v1"
+	corev1 "k8s.io/api/core/v1"
 	k8serr "k8s.io/apimachinery/pkg/api/errors"
 	metav1 "k8s.io/apimachinery/pkg/apis/meta/v1"
 	"k8s.io/apimachinery/pkg/apis/meta/v1/unstructured"
@@ -99,6 +101,10 @@ func creationTestSuite(t *testing.T) {
 			err = testCtx.testDefaultModelRegistryCertAvailable()
 			require.NoError(t, err, "error getting default cert secret for ModelRegistry")
 		})
+		t.Run("Validate trusted CA bundle", func(t *testing.T) {
+			err = testCtx.testTrustedCABundle()
+			require.NoError(t, err, "error validating trusted CA bundle")
+		})
 		t.Run("Validate model registry servicemeshmember available", func(t *testing.T) {
 			err = testCtx.testMRServiceMeshMember()
 			require.NoError(t, err, "error getting servicemeshmember for Model Registry")
@@ -444,6 +450,46 @@ func (tc *testContext) testDefaultCertsAvailable() error {
 	return nil
 }
 
+func (tc *testContext) testTrustedCABundle() error {
+	CAConfigMapName := "odh-trusted-ca-bundle"
+	CADataFieldName := "odh-ca-bundle.crt"
+
+	if tc.testDSCI.Spec.TrustedCABundle.ManagementState == operatorv1.Managed {
+		foundConfigMap := &corev1.ConfigMap{}
+		err := tc.customClient.Get(tc.ctx, client.ObjectKey{
+			Name:      CAConfigMapName,
+			Namespace: tc.testDSCI.Spec.ApplicationsNamespace,
+		}, foundConfigMap)
+
+		if err != nil {
+			return fmt.Errorf("Config map not found, %w", err)
+		}
+
+		checkNewline := strings.HasSuffix(foundConfigMap.Data[CADataFieldName], "\n")
+
+		if checkNewline == false {
+			fmt.Print("Newline not found at the end of configmap")
+		}
+
+		if strings.TrimSpace(foundConfigMap.Data[CADataFieldName]) != tc.testDSCI.Spec.TrustedCABundle.CustomCABundle {
+			return fmt.Errorf("odh-trusted-ca-bundle in config map does not match with DSCI's TrustedCABundle.CustomCABundle, needs update: %w", err)
+		}
+	} else {
+		foundConfigMap := &corev1.ConfigMap{}
+		err := tc.customClient.Get(tc.ctx, client.ObjectKey{
+			Name:      CAConfigMapName,
+			Namespace: tc.testDSCI.Spec.ApplicationsNamespace,
+		}, foundConfigMap)
+
+		if k8serr.IsNotFound(err) {
+			fmt.Printf("Config map not found in the namespace")
+		} else {
+			return fmt.Errorf("failed to validate trusted CA bundle %w", err)
+		}
+	}
+	return nil
+}
+
 func (tc *testContext) testDefaultModelRegistryCertAvailable() error {
 	// return if MR is not set to Managed
 	if tc.testDsc.Spec.Components.ModelRegistry.ManagementState != operatorv1.Managed {
diff --git a/tests/e2e/helper_test.go b/tests/e2e/helper_test.go
index 62d9e92b24a..f6b021c92e4 100644
--- a/tests/e2e/helper_test.go
+++ b/tests/e2e/helper_test.go
@@ -94,7 +94,11 @@ func setupDSCICR(name string) *dsciv1.DSCInitialization {
 			},
 			TrustedCABundle: &dsciv1.TrustedCABundleSpec{
 				ManagementState: "Managed",
-				CustomCABundle:  "",
+				CustomCABundle: `-----BEGIN CERTIFICATE-----
+      MIIFVjCCAz6gAwIBAgIUQ+NxE9izWRRdt86M/TX9b7wFjUUwDQYJKoZIhvcNAQEL
+     ...
+      IrrVQJLuM7IjWcmOvFjai57QGfIvWcaMY1q6n6MLsLOaXLoRuBLpDLvPbmyAhykU
+      ------END ------`,
 			},
 			ServiceMesh: &infrav1.ServiceMeshSpec{
 				ControlPlane: infrav1.ControlPlaneSpec{