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The purpose of daoSPIM system is imaging a sample inside microfluidic device using light-sheet illumination and dual-view detection . The design allows simultaneous imaging from two directions (views) using a single camera sensor, by combining the two optical paths into one. This is achieved by using a knife-edge mirror (M-prism) between the arms. The figure below shows simplified arrangement in 3d (a), detailed optical layout (b), and a zoom-in of detection and excitation light rays as they pass through the glass coverslip (c).
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