TSH_background.Rmd

# Background

The GSP Neonatal hTSH assay is a solid phase, two-site
fluoroimmunometric assay based on the direct sandwich technique in
which two monoclonal antibodies (derived from mice) are directed
against two separate antigenic determinants on the hTSH
molecule. Calibrators controls and patient specimens containing hTSH
are reacted simultaneously with immobilized monoclonal antibodies
directed against a specific antigenic site on the B hTSH subunit and
europium-labeled monoclonal antibodies (directed against a different
antigenic site located partly on the B subunit and partly on the A
subunit) in assay buffer. The assay buffer elutes hTSH from the dried
blood spots on the filter paper disks. The complete assay requires
only one incubation step.

DELFIA Inducer dissociates europium ions from the labeled antibody
into solution where they form highly fluorescent chelates with
components of DELFIA Inducer. The fluorescence in each well is then
measured. The fluorescence of each sample is proportional to the
concentration of hTSH in the sample.