diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index a90a732bfe..5ff5afd864 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -19,7 +19,7 @@ repos:
         alias: ec
 
   - repo: https://github.com/pre-commit/mirrors-mypy
-    rev: "v1.9.0"
+    rev: "v1.10.0"
     hooks:
       - id: mypy
         additional_dependencies:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 0b4931a639..08ef6ed61e 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -59,6 +59,7 @@
 - Update gitpod/workspace-base Docker digest to 0af257e ([#2919](https://github.com/nf-core/tools/pull/2919))
 - Update GitHub Actions ([#2927](https://github.com/nf-core/tools/pull/2927))
 - Update gitpod/workspace-base Docker digest to 5aeb24f ([#2929](https://github.com/nf-core/tools/pull/2929))
+- Update pre-commit hook pre-commit/mirrors-mypy to v1.10.0 ([#2933](https://github.com/nf-core/tools/pull/2933))
 
 ## [v2.13.1 - Tin Puppy Patch](https://github.com/nf-core/tools/releases/tag/2.13) - [2024-02-29]