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Feature request: Remove unfixable reads #15

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Shellfishgene opened this issue Aug 7, 2019 · 4 comments
Open

Feature request: Remove unfixable reads #15

Shellfishgene opened this issue Aug 7, 2019 · 4 comments

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@Shellfishgene
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Hi! It would be nice to have an option to remove unfixable reads (and their pairs) from the output.

@jamlover
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Hi. I don't have anything to do with Rcorrector but here's a link to a few tools including a script that will do what you requested:
https://github.com/harvardinformatics/TranscriptomeAssemblyTools

Specifically you want the script FilterUncorrectabledPEfastq.py

I found the link to the tools while checking this out:
https://informatics.fas.harvard.edu/best-practices-for-de-novo-transcriptome-assembly-with-trinity.html

Perhaps you've already moved on but in case you haven't, hope that helps.

@Shellfishgene
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Thanks, I've already found that one also and have been using it. I just thought that should be easy to implement in Rcorrector so you wouln't have to go through the files twice.

@jamlover
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Agreed.

@mourisl
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mourisl commented Aug 21, 2019

Thanks for the suggestions. But I think for the unfixed reads, they might be from low expressed alternative-spliced transcripts. So including this option could make "removing“ be used without caution, and lose some positive reads.

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3 participants