import os import subprocess from platform import system from setuptools import setup current_dir = os.getcwd() os.system('python3 -m pip install --user --upgrade cutadapt') porechop_setup = f'{current_dir}/modules/Porechop/setup.py' os.system(f'python3 {porechop_setup} install') os.system('pip3 install nanopack') os.system('pip3 install pycoQC') os.system('pip3 install cutadapt') os.system('pip3 install biopython') os.system('pip3 install numpy') any2fasta_dir = f'{current_dir}/modules/any2fasta/' os.system(f'chmod +x {any2fasta_dir}') os.system('git clone https://github.com/gmod/jbrowse modules/jbrowse') subprocess.call(['cd', f'{current_dir}/modules/jbrowse', 'git checkout 1.16.11-release', './setup.sh']) os.system('git clone https://github.com/rrwick/Bandage.git modules/Bandage') os.path.join(os.environ.get('QT_SELECT'), '=5') subprocess.call(['cd', f'{current_dir}/modules/Bandage', 'qmake', 'make']) os.system('git clone https://github.com/sanger-pathogens/Artemis.git modules/Artemis') subprocess.call(['cd', f'{current_dir}/modules/Artemis', 'mvn validate', 'mvn clean package']) os.system('git clone https://github.com/arq5x/bedtools2.git modules/bedtools2') subprocess.call(['cd', f'{current_dir}/modules/bedtools2', 'make']) os.system('git clone https://github.com/brinkmanlab/islandpath.git modules/islandpath') os.system('git clone https://github.com/CRISPRlab/CRISPRviz.git modules/CRISPRviz') os.system('git clone https://github.com/ablab/quast.git modules/quast') os.system('git clone https://github.com/PacificBiosciences/barcoding.git modules/barcoding') os.system('git clone https://github.com/marbl/canu.git modules/canu') os.system('./modules/canu/src make') flye_make = f'{current_dir}/modules/Flye/make' os.system(flye_make) unicycler_setup = f'{current_dir}/modules/Unicycler/setup.py' os.system(f'python3 {unicycler_setup} install') nanopolish_make = f'{current_dir}/modules/nanopolish/make' os.system(nanopolish_make) os.system('cpan Bio::Perl Data::Dumper Log:Log4perl Config::Simple Moose MooseX::Singleton') prokka_setup = f'{current_dir}/modules/prokka/bin/prokka' os.system(f'{prokka_setup} --setupdb') os.system('python3 ./modules/Kleborate/setup.py') os.system(f'{current_dir}/modules/abricate/bin/abricate --check') os.system(f'{current_dir}/modules/abricate/bin/abricate --setupdb') os.system(f'{current_dir}/modules/abricate/bin/abricate {current_dir}/modules/abricate/test/assembly.fa') os.system(f'{current_dir}/modules/Prodigal/make install') os.system(f'{current_dir}/modules/hmmer-3.3.2/./configure --prefix {current_dir}/modules/hmmer-3.3.2') subprocess.call(['cd', f'{current_dir}/modules/hmmer-3.3.2', 'make']) subprocess.call(['cd', f'{current_dir}/modules/hmmer-3.3.2', 'make check']) subprocess.call(['cd', f'{current_dir}/modules/hmmer-3.3.2', 'make install']) subprocess.call(['cd', f'{current_dir}/modules/phigaro', 'phigaro-setup --no-updatedb']) os.path.join(os.environ.get('PATH'), ':{current_dir}/modules/barrnap/bin:{current_dir}/modules/CRISPRviz/bin:{current_dir}/modules/quast/quast.py') os.system(f'java -jar {current_dir}/modules/pilon-1.24.jar --version') os.system('which crisprviz.sh') os.system(f'cd {current_dir}/modules/quast && ./setup.py install') os.system(f'cd {current_dir}/modules/quast && ./setup.py test') os.system(f'mkdir {current_dir}/dataDB') os.system('python3 ./back-end/app.py flask run')