A command-line tool that find optimized PCR-primer for a given fasta file region.
Results of this tool follows somes rules:
- 17 to 25 nucleotids-long primer
- Primer has to end with a C or G
- Both primer need to have the same hybridation temps
To be implemented:
- addition of restriction sites
- addition of promotor
python main.py -f filename.fasta -b [begining of region interest] -e [end of region of interest]Example:
python main.py -f test.fasta -b 10 -e 200(python3)
