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<h2>Contribution</h2>
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<table class="table table-hover table-bordered">
<thead>
<tr>
<th scope="col">Part Number</th>
<th scope="col">Part Name</th>
<th scope="col">Type</th>
<th scope="col">Description</th>
<th scope="col">Attribution</th>
<th scope="col">Length (bp)</th>
</tr>
</thead>
<tbody>
<tr>
<th scope="row">BBa_K3979009</th>
<td>Chi B</td>
<td>Basic</td>
<td>Chitinase B Coding sequence [<i><i>Serratia marcescens</i></i> strain QMB1466] <b>Codon optimised wild type bacterial chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>1524</td>
</tr>
<tr>
<th scope="row">BBa_K3979010</th>
<td>ChiA</td>
<td>Basic</td>
<td>Chitinase A precursor Coding Sequence[<i><i>Amycolatopsis orientalis</i></i> strain B-37] <b>Codon optimised wild type plant chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>975</td>
<tr>
<th scope="row">BBa_K3979005</th>
<td>chitinase II precursor</td>
<td>Basic</td>
<td>Chitinase II precursor Coding Sequence[<i>Triticum aestivum</i> cultivar Sumai 3] <b>Codon optimised wild type plant chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>719</td>
</tr>
<tr>
<th scope="row">BBa_K3979006</th>
<td>Barley Chitinase</td>
<td>Basic</td>
<td>Barley Chitinase[<i>Hordeum vulgare</i> cultivar NK1558] <b>Codon optimised wild type plant chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>981</td>
</tr>
<tr>
<th scope="row">BBa_K3979004</th>
<td>BC1</td>
<td>Basic</td>
<td>Chitinase combo Coding Sequence
of ChiB (<i><i>Serratia marcescens</i></i> strain QMB1466) and ChiA (<i><i>Pseudoalteromonas</i>sp. DL-6</i>)<b>Codon optimised chimeric bacterial chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>1818</td>
</tr>
<tr>
<th scope="row">BBa_K3979003</th>
<td>BC2</td>
<td>Basic</td>
<td>Chitinase combo Coding Sequence
of ChiB (<i><i>Serratia marcescens</i></i> strain QMB1466) and ChiA (<i><i>Amycolatopsis orientalis</i> B-37</i>)<b>Codon optimised chimeric bacterial chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>1161</td>
</tr>
<tr>
<th scope="row">BBa_K3979007</th>
<td>PC1</td>
<td>Basic</td>
<td>Chitinase combo Coding Sequence
of Chitinase II precursor[<i>Triticum aestivum</i>] and Endochitinase 1 (<i>Hordeum vulgare cultivar NK1558 </i>)<b>Codon optimised chimeric plant chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>1884</td>
</tr>
<tr>
<th scope="row">BBa_K3979008</th>
<td>PC2</td>
<td>Basic</td>
<td>Chitinase combo Coding Sequence
of Chitinase II precursor[<i>Triticum aestivum</i> cultivar Sumai 3] and Endochitinase 1 (<i>Hordeum vulgare cultivar NK1558 </i>)<b>Codon optimised chimeric plant chitinase</b></td>
<td>Abhishek Raghunathan and Akshay J</td>
<td>1035</td>
</tr>
</tbody>
</table>
</div>
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</div>
<!--
<section class="sample-text-area">
<div class="container box_1170">
<h2 class="text-center">Bacterial Chimeric Chitinase Design</h2>
<br>
<p class="pl-20 pr-20">
We designed two chimeric bacteria chitinases from three bacterial sources: <i>Pseudoalteromonas</i> sp DL-6, <i><i>Amycolatopsis orientalis</i></i>, and <i><i>Serratia marcescens</i></i>.
</p>
<p class="pl-20 pr-20">
<b>First chimeric combination:</b> We replaced the catalytic domain of <i><i>Serratia marcescens</i></i> with the catalytic domain of <i>Pseudoalteromonas</i> following the basic structure of <i>Serratia</i> chitinase.
</p>
<h4 class="pl-20 pr-20">Basic Outline</h4>
<p class="pl-20 pr-20">
Amino Acids in the beginning of <i>Serratia</i> ChiB natural sequence — CBD of <i>S. marcescens</i> Chi B — CD of <i>Pseudoalteromonas</i> Chi A — Chi B natural linker between CD and CBD of <i>S. marcescens</i> — CBD of <i>S. marcescens</i>
</p>
</div>
<div class="image-block, text-center">
<img src="assets/img/project/BC1_Domain_Structure.png" alt="sample image" class="img-fluid">
<p><strong>Figure 1.</strong> Unannotated image</p>
</div>
<div class="container box_1170">
<br><p class="pl-20 pr-20">
<b>Second chimeric combination:</b> We chose chitinase from Amycolaptosis orientalis which lacks a chitin-binding domain. So we added the chitin-binding domain of S. marcescens Chi B in the C-terminal of the <i>Amycolatopsis orientalis</i> chitinase.
</p>
<h4 class="pl-20 pr-20">Basic Outline</h4>
<p class="pl-20 pr-20">
Chitinase seq. of <i>Amycolatopsis orientalis</i> — CBD of S. marcescens Chi B
</p>
</div>
<div class="image-block, text-center">
<img src="assets/img/project/BC2_Domain_Structure.png" alt="sample image" class="img-fluid">
<p><strong>Figure 1.</strong> Unannotated image</p>
</div>
<br>
<br>
<br>
<div class="container box_1170">
<h2 class="text-center">Plant Chimeric Chitinase Design</h2>
<br>
<p class="pl-20 pr-20">
Apart from bacterial chitinases, we chose plant chitinases based on suggestions from structural biology experts since these chitinases would have evolved with more efficient chitinolytic activity. We designed two chimeric plant chitinase combinations using chitinases from two sources: <i>Triticum aestivum</i> (Wheat) and <i>Hordeum vulgare</i> (Barley). The cloning and the succeeding downstream assay experiments could not be performed due to lack of time. Both of these plant chitinases show efficient chitinolytic activity and retain activity at body temperature and pH range.
</p>
<p class="pl-20 pr-20">
<b>First chimeric combination:</b> We joined the sequences of both barley and wheat chitinases using common (GGGS)\(_3\) linkers. Wheat chitinase doesn’t have a chitin-binding domain, so we added barley CBD in its C-terminal in the combination.
</p>
<h4 class="pl-20 pr-20">Basic Outline</h4>
<p class="pl-20 pr-20">
AA seq before Barley CBD — Barley CBD — Barley CD — AA after CD barley — (GGGS)\(_{3}\) — AA seq before wheat CD — Wheat CD — natural linker from barley — Barley CBD
</p>
</div>
<div class="image-block, text-center">
<img src="assets/img/project/PC1_Domain_Structure.png" alt="sample image" class="img-fluid">
<p><strong>Figure 1.</strong> Unannotated image</p>
</div>
<div class="container box_1170">
<br><p class="pl-20 pr-20">
<b>Second chimeric combination:</b> We created a chimeric chitinase with a wheat catalytic domain flanked by two barley chitin-binding domains.
</p>
<h4 class="pl-20 pr-20">Basic Outline</h4>
<p class="pl-20 pr-20">
AA before barley chitinase — Barley CBD — natural linker from barley — Wheat CD — natural linker from Barley — Barley CBD
</p>
</div>
<div class="image-block, text-center">
<img src="assets/img/project/PC2_Domain_Structure.png" alt="sample image" class="img-fluid">
<p><strong>Figure 1.</strong> Unannotated image</p>
</div>-->
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<!--<p class="text-center">The sequences that we created are codon-optimized in order to clone and express them in <i>E. coli</i>.</p>-->
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