diff --git a/packages/vite/src/node/plugins/define.ts b/packages/vite/src/node/plugins/define.ts
index 39d7b6bf8f3f5e..2b7a7cd8d508d8 100644
--- a/packages/vite/src/node/plugins/define.ts
+++ b/packages/vite/src/node/plugins/define.ts
@@ -1,5 +1,4 @@
 import { transform } from 'esbuild'
-import { TraceMap, decodedMap, encodedMap } from '@jridgewell/trace-mapping'
 import type { ResolvedConfig } from '../config'
 import type { Plugin } from '../plugin'
 import { escapeRegex } from '../utils'
@@ -201,26 +200,6 @@ export async function replaceDefine(
         : true,
   })
 
-  // remove esbuild's <define:...> source entries
-  // since they would confuse source map remapping/collapsing which expects a single source
-  if (result.map.includes('<define:')) {
-    const originalMap = new TraceMap(result.map)
-    if (originalMap.sources.length >= 2) {
-      const sourceIndex = originalMap.sources.indexOf(id)
-      const decoded = decodedMap(originalMap)
-      decoded.sources = [id]
-      decoded.mappings = decoded.mappings.map((segments) =>
-        segments.filter((segment) => {
-          // modify and filter
-          const index = segment[1]
-          segment[1] = 0
-          return index === sourceIndex
-        }),
-      )
-      result.map = JSON.stringify(encodedMap(new TraceMap(decoded as any)))
-    }
-  }
-
   return {
     code: result.code,
     map: result.map || null,