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im running pvacsplice using docker images pvactools:5.1.0 and 5.0.1 and got this error:
Warning: The above variant/transcript/gene combination is present in the junctions file, but not in the VEP-annotated VCF. Skipping.
Traceback (most recent call last):
File "/usr/local/bin/pvacsplice", line 8, in <module>
sys.exit(main())
File "/usr/local/lib/python3.7/site-packages/pvactools/tools/pvacsplice/main.py", line 117, in main
args[0].func.main(args[1])
File "/usr/local/lib/python3.7/site-packages/pvactools/tools/pvacsplice/run.py", line 149, in main
pipeline.execute()
File "/usr/local/lib/python3.7/site-packages/pvactools/lib/splice_pipeline.py", line 46, in execute
self.junction_to_fasta()
File "/usr/local/lib/python3.7/site-packages/pvactools/lib/splice_pipeline.py", line 149, in junction_to_fasta
combined_df = self.combine_inputs()
File "/usr/local/lib/python3.7/site-packages/pvactools/lib/splice_pipeline.py", line 143, in combine_inputs
sys.exit("Combined dataset is empty. Aborting.")
NameError: name 'sys' is not defined
I know that the variant is indeed present in the input VEP-annotated VCF. So pvacsplice wasnt running probably because of the missing 'sys' package.
command:
pvacsplice run -g -k -t 4 --normal-sample-name WT-B6-SPLEEN splice_junction.tsv P6-WCMC H-2-Kb,H-2-Db,H2-IAb all /storage1/fs1/tfehnige/Active/fehniger_lymphoma/SPORE/P1P6/wdl_runs/P6-WCMC/pvacsplice/i10e10 annotated_filtered_NoExistingVar.vcf.gz Mus_musculus.GRCm39.dna.primary_assembly.fa Mus_musculus.GRCm39.113.gtf
The text was updated successfully, but these errors were encountered:
mhoang22
changed the title
missing sys package in pvactools docker image 5.0.1 and 5.1.0
cant run pvacsplice on non-human species (when using pvactools docker image 5.0.1 and 5.1.0)
Feb 6, 2025
im running pvacsplice using docker images pvactools:5.1.0 and 5.0.1 and got this error:
I know that the variant is indeed present in the input VEP-annotated VCF. So pvacsplice wasnt running probably because of the missing 'sys' package.
command:
The text was updated successfully, but these errors were encountered: