diff --git a/doc/classes/ProjectSettings.xml b/doc/classes/ProjectSettings.xml
index ce36a0336d29..720a4f6a47a3 100644
--- a/doc/classes/ProjectSettings.xml
+++ b/doc/classes/ProjectSettings.xml
@@ -1964,6 +1964,9 @@
 		<member name="navigation/3d/default_link_connection_radius" type="float" setter="" getter="" default="1.0">
 			Default link connection radius for 3D navigation maps. See [method NavigationServer3D.map_set_link_connection_radius].
 		</member>
+		<member name="navigation/3d/default_up" type="Vector3" setter="" getter="" default="Vector3(0, 1, 0)">
+			Default up orientation for 3D navigation maps. See [method NavigationServer3D.map_set_up].
+		</member>
 		<member name="navigation/3d/use_edge_connections" type="bool" setter="" getter="" default="true">
 			If enabled 3D navigation regions will use edge connections to connect with other navigation regions within proximity of the navigation map edge connection margin. This setting only affects World3D default navigation maps.
 		</member>
diff --git a/scene/resources/world_3d.cpp b/scene/resources/world_3d.cpp
index 297d219caf36..b8646c538712 100644
--- a/scene/resources/world_3d.cpp
+++ b/scene/resources/world_3d.cpp
@@ -68,6 +68,7 @@ RID World3D::get_navigation_map() const {
 		NavigationServer3D::get_singleton()->map_set_active(navigation_map, true);
 		NavigationServer3D::get_singleton()->map_set_cell_size(navigation_map, GLOBAL_GET("navigation/3d/default_cell_size"));
 		NavigationServer3D::get_singleton()->map_set_cell_height(navigation_map, GLOBAL_GET("navigation/3d/default_cell_height"));
+		NavigationServer3D::get_singleton()->map_set_up(navigation_map, GLOBAL_GET("navigation/3d/default_up"));
 		NavigationServer3D::get_singleton()->map_set_use_edge_connections(navigation_map, GLOBAL_GET("navigation/3d/use_edge_connections"));
 		NavigationServer3D::get_singleton()->map_set_edge_connection_margin(navigation_map, GLOBAL_GET("navigation/3d/default_edge_connection_margin"));
 		NavigationServer3D::get_singleton()->map_set_link_connection_radius(navigation_map, GLOBAL_GET("navigation/3d/default_link_connection_radius"));
diff --git a/servers/navigation_server_3d.cpp b/servers/navigation_server_3d.cpp
index 2536ce719d4b..2d4bc018f029 100644
--- a/servers/navigation_server_3d.cpp
+++ b/servers/navigation_server_3d.cpp
@@ -186,6 +186,7 @@ NavigationServer3D::NavigationServer3D() {
 
 	GLOBAL_DEF_BASIC("navigation/3d/default_cell_size", 0.25);
 	GLOBAL_DEF_BASIC("navigation/3d/default_cell_height", 0.25);
+	GLOBAL_DEF("navigation/3d/default_up", Vector3(0, 1, 0));
 	GLOBAL_DEF("navigation/3d/use_edge_connections", true);
 	GLOBAL_DEF_BASIC("navigation/3d/default_edge_connection_margin", 0.25);
 	GLOBAL_DEF_BASIC("navigation/3d/default_link_connection_radius", 1.0);