diff --git a/erpnext/public/js/controllers/transaction.js b/erpnext/public/js/controllers/transaction.js
index edc4b06dca19b..382460bb19620 100644
--- a/erpnext/public/js/controllers/transaction.js
+++ b/erpnext/public/js/controllers/transaction.js
@@ -423,7 +423,7 @@ erpnext.TransactionController = class TransactionController extends erpnext.taxe
 		item.barcode = null;
 
 
-		if(item.item_code || item.barcode || item.serial_no) {
+		if(item.item_code || item.serial_no) {
 			if(!this.validate_company_and_party()) {
 				this.frm.fields_dict["items"].grid.grid_rows[item.idx - 1].remove();
 			} else {
diff --git a/erpnext/public/js/utils/barcode_scanner.js b/erpnext/public/js/utils/barcode_scanner.js
index 943db07705e01..a6bff2c148dfd 100644
--- a/erpnext/public/js/utils/barcode_scanner.js
+++ b/erpnext/public/js/utils/barcode_scanner.js
@@ -9,6 +9,7 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 		this.barcode_field = opts.barcode_field || "barcode";
 		this.serial_no_field = opts.serial_no_field || "serial_no";
 		this.batch_no_field = opts.batch_no_field || "batch_no";
+		this.uom_field = opts.uom_field || "uom";
 		this.qty_field = opts.qty_field || "qty";
 		// field name on row which defines max quantity to be scanned e.g. picklist
 		this.max_qty_field = opts.max_qty_field;
@@ -26,6 +27,7 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 		//     bar_code: "123456", // present if barcode was scanned
 		//     batch_no: "LOT12", // present if batch was scanned
 		//     serial_no: "987XYZ", // present if serial no was scanned
+		//     uom: "Kg", // present if barcode UOM is different from default
 		// }
 		this.scan_api = opts.scan_api || "erpnext.stock.utils.scan_barcode";
 	}
@@ -67,9 +69,9 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 		return new Promise(resolve => {
 			let cur_grid = this.frm.fields_dict[this.items_table_name].grid;
 
-			const {item_code, barcode, batch_no, serial_no} = data;
+			const {item_code, barcode, batch_no, serial_no, uom} = data;
 
-			let row = this.get_row_to_modify_on_scan(item_code, batch_no);
+			let row = this.get_row_to_modify_on_scan(item_code, batch_no, uom);
 
 			if (!row) {
 				if (this.dont_allow_new_row) {
@@ -90,10 +92,11 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 			}
 
 			frappe.run_serially([
-				() => this.set_selector_trigger_flag(row, data),
+				() => this.set_selector_trigger_flag(data),
 				() => this.set_item(row, item_code).then(qty => {
 					this.show_scan_message(row.idx, row.item_code, qty);
 				}),
+				() => this.set_barcode_uom(row, uom),
 				() => this.set_serial_no(row, serial_no),
 				() => this.set_batch_no(row, batch_no),
 				() => this.set_barcode(row, barcode),
@@ -106,7 +109,7 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 
 	// batch and serial selector is reduandant when all info can be added by scan
 	// this flag on item row is used by transaction.js to avoid triggering selector
-	set_selector_trigger_flag(row, data) {
+	set_selector_trigger_flag(data) {
 		const {batch_no, serial_no, has_batch_no, has_serial_no} = data;
 
 		const require_selecting_batch = has_batch_no && !batch_no;
@@ -154,6 +157,12 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 		}
 	}
 
+	async set_barcode_uom(row, uom) {
+		if (uom && frappe.meta.has_field(row.doctype, this.uom_field)) {
+			await frappe.model.set_value(row.doctype, row.name, this.uom_field, uom);
+		}
+	}
+
 	async set_batch_no(row, batch_no) {
 		if (batch_no && frappe.meta.has_field(row.doctype, this.batch_no_field)) {
 			await frappe.model.set_value(row.doctype, row.name, this.batch_no_field, batch_no);
@@ -184,7 +193,7 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 		return is_duplicate;
 	}
 
-	get_row_to_modify_on_scan(item_code, batch_no) {
+	get_row_to_modify_on_scan(item_code, batch_no, uom) {
 		let cur_grid = this.frm.fields_dict[this.items_table_name].grid;
 
 		// Check if batch is scanned and table has batch no field
@@ -193,10 +202,12 @@ erpnext.utils.BarcodeScanner = class BarcodeScanner {
 
 		const matching_row = (row) => {
 			const item_match = row.item_code == item_code;
-			const batch_match = row.batch_no == batch_no;
+			const batch_match = row[this.batch_no_field] == batch_no;
+			const uom_match = !uom || row[this.uom_field] == uom;
 			const qty_in_limit = flt(row[this.qty_field]) < flt(row[this.max_qty_field]);
 
 			return item_match
+				&& uom_match
 				&& (!is_batch_no_scan || batch_match)
 				&& (!check_max_qty || qty_in_limit)
 		}
diff --git a/erpnext/stock/doctype/item_barcode/item_barcode.json b/erpnext/stock/doctype/item_barcode/item_barcode.json
index eef70c95d05c7..c7ed673993594 100644
--- a/erpnext/stock/doctype/item_barcode/item_barcode.json
+++ b/erpnext/stock/doctype/item_barcode/item_barcode.json
@@ -6,7 +6,8 @@
  "engine": "InnoDB",
  "field_order": [
   "barcode",
-  "barcode_type"
+  "barcode_type",
+  "uom"
  ],
  "fields": [
   {
@@ -24,11 +25,17 @@
    "in_list_view": 1,
    "label": "Barcode Type",
    "options": "\nEAN\nUPC-A"
+  },
+  {
+   "fieldname": "uom",
+   "fieldtype": "Link",
+   "label": "UOM",
+   "options": "UOM"
   }
  ],
  "istable": 1,
  "links": [],
- "modified": "2022-04-01 05:54:27.314030",
+ "modified": "2022-05-12 08:11:10.411590",
  "modified_by": "Administrator",
  "module": "Stock",
  "name": "Item Barcode",
diff --git a/erpnext/stock/utils.py b/erpnext/stock/utils.py
index 2120304097b0b..6d8fdaa40420e 100644
--- a/erpnext/stock/utils.py
+++ b/erpnext/stock/utils.py
@@ -558,7 +558,7 @@ def scan_barcode(search_value: str) -> Dict[str, Optional[str]]:
 	barcode_data = frappe.db.get_value(
 		"Item Barcode",
 		{"barcode": search_value},
-		["barcode", "parent as item_code"],
+		["barcode", "parent as item_code", "uom"],
 		as_dict=True,
 	)
 	if barcode_data: