diff --git a/README.md b/README.md index 6e66eff..415ea57 100644 --- a/README.md +++ b/README.md @@ -25,9 +25,65 @@ Because the archived files are demultiplexed, the initial demultiplexing step in * Download the v3 genome [here](https://phytozome.jgi.doe.gov/pz/portal.html#!info?alias=Org_Ptrichocarpa_er). * Create an searchable database, using `bowtie2-build`, and move the indexed files to `data/referenceDBs/Ptri.v.3.db`. -* Software dependencies can be found in the individual scripts in the `code/` folder. +* Software dependencies (versions used). + * bowtie2 (v2.3.5) + * cutadapt (v2.7) + * pheniqs (v2.0.4) + * R (3.6.2) + * SeqPurge (v2019_11) +* R packages + * betareg (v3.1-3) + * Biostrings (v2.54.0) + * cowplot (v1.0.0) + * dada2 (v1.15) + * DECIPHER (v2.14) + * doMC (v1.3.6) + * emmeans (v1.4.4) + * foreach (v1.4.8) + * ggbeeswarm (v0.6.0) + * ggtext (v0.1.0) + * ggthemes (v4.2.0) + * ggvegan (v0.1-0) + * lmtest (v0.9-37) + * magrittr (v1.5) + * MASS (v7.3-51.5) + * mvabund (v4.0.1), + * phyloseq (v1.30.0) + * ShortRead (v1.44.3) + * tidyverse (v1.3) + * vegan (v2.5-6) +### Descriptions of code + * biasEstimates.R + * Use mock community data to estimate taxon specific biases in the Illumina sequence data. + * colors.R + * Define color paletts used in figures. + * compile.R + * Process denoised Illumina data to prepare for analysis. Includes removing host contamination, collapsing denoised sequence variants to 99% OTUs, identifying focal taxa, and removing samples with poor coverage. + * demux.config.json + * Define instructions for demultiplexing with Pheniqs. + * denoise.R + * Denoise Illumina data to identify amplicon sequence variants with DADA2. + * jsdModels.R + * Multvariate test using joint species distribution models with mvabund. + * mapFigS1.R + * make map showing geographic origins of *P. trichocarpa* genotypes. + * ordinations.R + * Visualize community data using distance-based redundancy analysis. + * priorityEffects.R + * Explore the bennefit of preemptive colonization for the fungal species used as early colonists in the immigration history treatments. + * Rfunctions.R + * Some custom R functions used by other scripts. + * rustAnalysis.R + * Test effects of experimental treatments on leaf rust severity and make corresponding figures. + * rustCor.R + * Explore possible correlations between relative abundance of foliar fungi and rust severity. + * trim.sh + * Process demultiplexed Illumina data to trim gene primers and read-through contamination. + * univariate.R + * Make figures showing responses of individual species to experimental treatments. +