diff --git a/examples/mysql/src/tracer.ts b/examples/mysql/src/tracer.ts
index 2ded0e2567..c80675f6b4 100644
--- a/examples/mysql/src/tracer.ts
+++ b/examples/mysql/src/tracer.ts
@@ -20,14 +20,15 @@ const EXPORTER = process.env.EXPORTER || '';
 export const setupTracing = (serviceName: string) => {
 
   //metrics:
-  const meterProvider = new MeterProvider()
   const metricExporter = new OTLPMetricExporter();
   const metricReader = new PeriodicExportingMetricReader({
     exporter: metricExporter,
     exportIntervalMillis: 100,
     exportTimeoutMillis: 100,
   });
-  meterProvider.addMetricReader(metricReader);
+  const meterProvider = new MeterProvider({
+    readers: [metricReader],
+  });
 
   //traces:
   const tracerProvider = new NodeTracerProvider({
diff --git a/plugins/node/instrumentation-runtime-node/test/event_loop_delay.test.ts b/plugins/node/instrumentation-runtime-node/test/event_loop_delay.test.ts
index 9571ff23c6..d77bfbdcfe 100644
--- a/plugins/node/instrumentation-runtime-node/test/event_loop_delay.test.ts
+++ b/plugins/node/instrumentation-runtime-node/test/event_loop_delay.test.ts
@@ -27,8 +27,9 @@ describe(`${ConventionalNamePrefix.NodeJs}.eventloop`, function () {
 
   beforeEach(() => {
     metricReader = new TestMetricReader();
-    meterProvider = new MeterProvider();
-    meterProvider.addMetricReader(metricReader);
+    meterProvider = new MeterProvider({
+      readers: [metricReader],
+    });
   });
 
   for (const metricName in metricNames) {
diff --git a/plugins/node/instrumentation-runtime-node/test/event_loop_time.test.ts b/plugins/node/instrumentation-runtime-node/test/event_loop_time.test.ts
index ac9a672ab9..7ee96150f3 100644
--- a/plugins/node/instrumentation-runtime-node/test/event_loop_time.test.ts
+++ b/plugins/node/instrumentation-runtime-node/test/event_loop_time.test.ts
@@ -29,8 +29,9 @@ describe(`${ConventionalNamePrefix.NodeJs}.${ATTR_NODEJS_EVENT_LOOP_TIME}`, func
 
   beforeEach(() => {
     metricReader = new TestMetricReader();
-    meterProvider = new MeterProvider();
-    meterProvider.addMetricReader(metricReader);
+    meterProvider = new MeterProvider({
+      readers: [metricReader],
+    });
   });
 
   it('should not export before being enabled', async function () {
diff --git a/plugins/node/instrumentation-runtime-node/test/event_loop_utilization.test.ts b/plugins/node/instrumentation-runtime-node/test/event_loop_utilization.test.ts
index e434557f20..3200dd0392 100644
--- a/plugins/node/instrumentation-runtime-node/test/event_loop_utilization.test.ts
+++ b/plugins/node/instrumentation-runtime-node/test/event_loop_utilization.test.ts
@@ -29,8 +29,9 @@ describe(`${ConventionalNamePrefix.NodeJs}.${ATTR_NODEJS_EVENT_LOOP_UTILIZATION}
 
   beforeEach(() => {
     metricReader = new TestMetricReader();
-    meterProvider = new MeterProvider();
-    meterProvider.addMetricReader(metricReader);
+    meterProvider = new MeterProvider({
+      readers: [metricReader],
+    });
   });
 
   it('should not export before being enabled', async function () {
diff --git a/plugins/node/instrumentation-runtime-node/test/heap_space_and_used.test.ts b/plugins/node/instrumentation-runtime-node/test/heap_space_and_used.test.ts
index 177834332f..43aae14344 100644
--- a/plugins/node/instrumentation-runtime-node/test/heap_space_and_used.test.ts
+++ b/plugins/node/instrumentation-runtime-node/test/heap_space_and_used.test.ts
@@ -31,8 +31,9 @@ describe('nodejs.heap_space', function () {
 
   beforeEach(() => {
     metricReader = new TestMetricReader();
-    meterProvider = new MeterProvider();
-    meterProvider.addMetricReader(metricReader);
+    meterProvider = new MeterProvider({
+      readers: [metricReader],
+    });
   });
 
   for (const metricName in metricNames) {
diff --git a/plugins/node/instrumentation-runtime-node/test/instrumentation.test.ts b/plugins/node/instrumentation-runtime-node/test/instrumentation.test.ts
index 34de947e15..f3ccf82a0e 100644
--- a/plugins/node/instrumentation-runtime-node/test/instrumentation.test.ts
+++ b/plugins/node/instrumentation-runtime-node/test/instrumentation.test.ts
@@ -27,8 +27,9 @@ describe('instrumentation', function () {
 
   beforeEach(() => {
     metricReader = new TestMetricReader();
-    meterProvider = new MeterProvider();
-    meterProvider.addMetricReader(metricReader);
+    meterProvider = new MeterProvider({
+      readers: [metricReader],
+    });
   });
 
   it('should not export before being enabled', async function () {
diff --git a/plugins/node/instrumentation-undici/test/metrics.test.ts b/plugins/node/instrumentation-undici/test/metrics.test.ts
index a903698385..de7a9ad5c7 100644
--- a/plugins/node/instrumentation-undici/test/metrics.test.ts
+++ b/plugins/node/instrumentation-undici/test/metrics.test.ts
@@ -37,12 +37,13 @@ describe('UndiciInstrumentation metrics tests', function () {
   const hostname = 'localhost';
   const mockServer = new MockServer();
   const provider = new NodeTracerProvider();
-  const meterProvider = new MeterProvider();
   const metricsMemoryExporter = new InMemoryMetricExporter(
     AggregationTemporality.DELTA
   );
   const metricReader = new MockMetricsReader(metricsMemoryExporter);
-  meterProvider.addMetricReader(metricReader);
+  const meterProvider = new MeterProvider({
+    readers: [metricReader],
+  });
 
   before(function (done) {
     // Do not test if the `fetch` global API is not available
diff --git a/plugins/node/opentelemetry-instrumentation-aws-lambda/test/integrations/lambda-handler.force-flush.test.ts b/plugins/node/opentelemetry-instrumentation-aws-lambda/test/integrations/lambda-handler.force-flush.test.ts
index 75cc30949e..8b68e71fe2 100644
--- a/plugins/node/opentelemetry-instrumentation-aws-lambda/test/integrations/lambda-handler.force-flush.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-aws-lambda/test/integrations/lambda-handler.force-flush.test.ts
@@ -152,10 +152,11 @@ describe('force flush', () => {
   });
 
   it('should force flush MeterProvider', async () => {
-    const provider = new MeterProvider();
-    provider.addMetricReader(
-      new PeriodicExportingMetricReader({ exporter: metricMemoryExporter })
-    );
+    const provider = new MeterProvider({
+      readers: [
+        new PeriodicExportingMetricReader({ exporter: metricMemoryExporter }),
+      ],
+    });
     let forceFlushed = false;
     const forceFlush = () =>
       new Promise<void>(resolve => {
@@ -197,10 +198,11 @@ describe('force flush', () => {
       });
     nodeTracerProvider.forceFlush = tracerForceFlush;
 
-    const meterProvider = new MeterProvider();
-    meterProvider.addMetricReader(
-      new PeriodicExportingMetricReader({ exporter: metricMemoryExporter })
-    );
+    const meterProvider = new MeterProvider({
+      readers: [
+        new PeriodicExportingMetricReader({ exporter: metricMemoryExporter }),
+      ],
+    });
     let meterForceFlushed = false;
     const meterForceFlush = () =>
       new Promise<void>(resolve => {
diff --git a/plugins/node/opentelemetry-instrumentation-mongodb/test/mongodb-v4-v5-v6.metrics.test.ts b/plugins/node/opentelemetry-instrumentation-mongodb/test/mongodb-v4-v5-v6.metrics.test.ts
index 9bb49cb45b..47e925ac14 100644
--- a/plugins/node/opentelemetry-instrumentation-mongodb/test/mongodb-v4-v5-v6.metrics.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-mongodb/test/mongodb-v4-v5-v6.metrics.test.ts
@@ -28,7 +28,6 @@ import {
   ResourceMetrics,
 } from '@opentelemetry/sdk-metrics';
 
-const otelTestingMeterProvider = new MeterProvider();
 const inMemoryMetricsExporter = new InMemoryMetricExporter(
   AggregationTemporality.CUMULATIVE
 );
@@ -37,6 +36,9 @@ const metricReader = new PeriodicExportingMetricReader({
   exportIntervalMillis: 100,
   exportTimeoutMillis: 100,
 });
+const otelTestingMeterProvider = new MeterProvider({
+  readers: [metricReader],
+});
 
 import { registerInstrumentationTesting } from '@opentelemetry/contrib-test-utils';
 const instrumentation = registerInstrumentationTesting(
@@ -85,7 +87,6 @@ describe('MongoDBInstrumentation-Metrics', () => {
   let collection: Collection;
 
   before(done => {
-    otelTestingMeterProvider.addMetricReader(metricReader);
     instrumentation?.setMeterProvider(otelTestingMeterProvider);
 
     shouldTest = true;
diff --git a/plugins/node/opentelemetry-instrumentation-mysql/test/index.metrics.test.ts b/plugins/node/opentelemetry-instrumentation-mysql/test/index.metrics.test.ts
index b6015d65a8..3661904b5b 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql/test/index.metrics.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql/test/index.metrics.test.ts
@@ -67,7 +67,6 @@ describe('mysql@2.x-Metrics', () => {
   const shouldTest = testMysql || testMysqlLocally; // Skips these tests if false (default)
 
   function initMeterProvider() {
-    otelTestingMeterProvider = new MeterProvider();
     inMemoryMetricsExporter = new InMemoryMetricExporter(
       AggregationTemporality.CUMULATIVE
     );
@@ -76,8 +75,10 @@ describe('mysql@2.x-Metrics', () => {
       exportIntervalMillis: 100,
       exportTimeoutMillis: 100,
     });
+    otelTestingMeterProvider = new MeterProvider({
+      readers: [metricReader],
+    });
 
-    otelTestingMeterProvider.addMetricReader(metricReader);
     instrumentation.setMeterProvider(otelTestingMeterProvider);
   }