diff --git a/ci/jenkins/test.sh b/ci/jenkins/test.sh
index 02f02def582..255f67960c2 100755
--- a/ci/jenkins/test.sh
+++ b/ci/jenkins/test.sh
@@ -532,7 +532,7 @@ function run_e2e {
     if [[ $FLEXIBLE_IPAM == true ]]; then
         go test -v antrea.io/antrea/test/e2e --logs-export-dir `pwd`/antrea-test-logs --provider remote -timeout=100m --prometheus --antrea-ipam
     else
-        go test -v antrea.io/antrea/test/e2e --logs-export-dir `pwd`/antrea-test-logs --provider remote -timeout=100m --prometheus
+        go test -v -run=TestTraceflow antrea.io/antrea/test/e2e --logs-export-dir `pwd`/antrea-test-logs --provider remote -timeout=100m --prometheus
     fi
     if [[ "$?" != "0" ]]; then
         TEST_FAILURE=true
diff --git a/test/e2e/traceflow_test.go b/test/e2e/traceflow_test.go
index 0b174057153..910098ca92e 100644
--- a/test/e2e/traceflow_test.go
+++ b/test/e2e/traceflow_test.go
@@ -15,6 +15,7 @@
 package e2e
 
 import (
+	"antrea.io/antrea/pkg/apis/controlplane/v1beta2"
 	"context"
 	"fmt"
 	"net"
@@ -1201,6 +1202,14 @@ func testTraceflowInterNode(t *testing.T, data *TestData) {
 		}
 	}()
 
+	antreaPod, err := data.getAntreaPodOnNode(node2)
+	if err = data.waitForNetworkpolicyRealized(antreaPod, allowAllEgressName, v1beta2.K8sNetworkPolicy); err != nil {
+		t.Fatal(err)
+	}
+	if err = data.waitForNetworkpolicyRealized(antreaPod, denyAllIngressName, v1beta2.K8sNetworkPolicy); err != nil {
+		t.Fatal(err)
+	}
+
 	testcases := []testcase{
 		{
 			name:      "interNodeTraceflowIPv4",
@@ -2165,6 +2174,27 @@ func (data *TestData) createNPAllowAllEgress(name string) (*networkingv1.Network
 	return data.createNetworkPolicy(name, spec)
 }
 
+// waitForNetworkpolicyRealized waits for the NetworkPolicy to be realized by the antrea-agent Pod.
+func (data *TestData) waitForNetworkpolicyRealized(pod string, networkpolicy string, npType v1beta2.NetworkPolicyType) error {
+	npOption := "K8sNP"
+	if npType == v1beta2.AntreaNetworkPolicy {
+		npOption = "ANP"
+	}
+	if err := wait.Poll(200*time.Millisecond, 5*time.Second, func() (bool, error) {
+		cmds := []string{"antctl", "get", "networkpolicy", "-S", networkpolicy, "-n", testNamespace, "-T", npOption}
+		stdout, stderr, err := runAntctl(pod, cmds, data)
+		if err != nil {
+			return false, fmt.Errorf("Error when executing antctl get NetworkPolicy, stdout: %s, stderr: %s, err: %v", stdout, stderr, err)
+		}
+		return strings.Contains(stdout, fmt.Sprintf("%s:%s/%s", npType, testNamespace, networkpolicy)), nil
+	}); err == wait.ErrWaitTimeout {
+		return fmt.Errorf("NetworkPolicy %s isn't realized in time", networkpolicy)
+	} else if err != nil {
+		return err
+	}
+	return nil
+}
+
 func runTestTraceflow(t *testing.T, data *TestData, tc testcase) {
 	switch tc.ipVersion {
 	case 4: