diff --git a/ports/atmel-samd/boards/arduino_mkr1300/mpconfigboard.mk b/ports/atmel-samd/boards/arduino_mkr1300/mpconfigboard.mk
index abbdbe87efe4c..c3d75202bffcc 100644
--- a/ports/atmel-samd/boards/arduino_mkr1300/mpconfigboard.mk
+++ b/ports/atmel-samd/boards/arduino_mkr1300/mpconfigboard.mk
@@ -9,5 +9,3 @@ CHIP_FAMILY = samd21
 INTERNAL_FLASH_FILESYSTEM = 1
 LONGINT_IMPL = NONE
 CIRCUITPY_FULL_BUILD = 0
-
-CIRCUITPY_RAINBOWIO = 0
diff --git a/ports/atmel-samd/boards/arduino_nano_33_iot/mpconfigboard.mk b/ports/atmel-samd/boards/arduino_nano_33_iot/mpconfigboard.mk
index a61e321b291e6..895c027ee5ee6 100644
--- a/ports/atmel-samd/boards/arduino_nano_33_iot/mpconfigboard.mk
+++ b/ports/atmel-samd/boards/arduino_nano_33_iot/mpconfigboard.mk
@@ -9,5 +9,3 @@ CHIP_FAMILY = samd21
 INTERNAL_FLASH_FILESYSTEM = 1
 LONGINT_IMPL = NONE
 CIRCUITPY_FULL_BUILD = 0
-
-CIRCUITPY_RAINBOWIO = 0
diff --git a/ports/atmel-samd/boards/arduino_zero/mpconfigboard.mk b/ports/atmel-samd/boards/arduino_zero/mpconfigboard.mk
index 58fd9ca6e47e0..5ee22c59ad4c1 100644
--- a/ports/atmel-samd/boards/arduino_zero/mpconfigboard.mk
+++ b/ports/atmel-samd/boards/arduino_zero/mpconfigboard.mk
@@ -9,5 +9,3 @@ CHIP_FAMILY = samd21
 INTERNAL_FLASH_FILESYSTEM = 1
 LONGINT_IMPL = NONE
 CIRCUITPY_FULL_BUILD = 0
-
-CIRCUITPY_RAINBOWIO = 0
diff --git a/ports/atmel-samd/boards/sensebox_mcu/mpconfigboard.mk b/ports/atmel-samd/boards/sensebox_mcu/mpconfigboard.mk
index f59483a608c63..bdfe26068de20 100644
--- a/ports/atmel-samd/boards/sensebox_mcu/mpconfigboard.mk
+++ b/ports/atmel-samd/boards/sensebox_mcu/mpconfigboard.mk
@@ -13,4 +13,3 @@ CIRCUITPY_FULL_BUILD = 0
 # There are many pin definitions on this board; it doesn't quite fit on very large translations.
 # Remove a couple of modules.
 CIRCUITPY_ONEWIREIO = 0
-CIRCUITPY_RAINBOWIO = 0
diff --git a/ports/nrf/boards/simmel/mpconfigboard.mk b/ports/nrf/boards/simmel/mpconfigboard.mk
index 82fc68a2359b7..992188b1d40e6 100644
--- a/ports/nrf/boards/simmel/mpconfigboard.mk
+++ b/ports/nrf/boards/simmel/mpconfigboard.mk
@@ -28,7 +28,6 @@ CIRCUITPY_NVM = 0
 CIRCUITPY_PIXELBUF  = 0
 CIRCUITPY_PULSEIO = 0
 CIRCUITPY_PWMIO = 1
-CIRCUITPY_RAINBOWIO = 0
 CIRCUITPY_RGBMATRIX = 0
 CIRCUITPY_ROTARYIO = 0
 CIRCUITPY_RTC = 1