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Whether the software supports fastq data from both DNAseq and RNAseq or not? #5

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xiezhan opened this issue Dec 4, 2017 · 1 comment

xiezhan commented Dec 4, 2017

Can I use this soft to call fusion gene from RNAseq data?
Could anyone give me some advice?

thanks so much !

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sfchen commented Dec 4, 2017

This tool is originally designed for detecting fusion from DNA-Seq data.

Theoretically it can be used for detecting fusion from short reads (Illumina / Lifetech etc) RNA-Seq data. But I haven't done a lot of testing.

False negative may happen in such case: the fused exon is too short, and its neighbour intron is too long.

For long reads RNA-Seq (PacBio / Nanopore etc) RNA-Seq data, please use other tools.

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