Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Screen for all known illumina adapters #7

Closed
ewels opened this issue Apr 16, 2020 · 1 comment · Fixed by #14
Closed

Screen for all known illumina adapters #7

ewels opened this issue Apr 16, 2020 · 1 comment · Fixed by #14
Assignees
Milestone

Comments

@ewels
Copy link

ewels commented Apr 16, 2020

It would be nice to be able to supply a file of all known illumina indexes to screen for unexpected contamination, even for low level pool contamination.

@remiolsen
Copy link
Member

remiolsen commented Oct 6, 2020

Suggestion after discussing this with @FranBonath is to do a count of barcodes using the same mapping procedure that already exists, with the following rules:

  1. Only use perfectly mapping barcodes (i.e. no indels)
  2. Exclude i5+i7 barcodes found in the samplesheet
  3. Output a list of 100 (500?) most abundant barcodes found

At first, use only the library constructs that are in the samplesheet. For instance single-index 8bp separately, from dual index 8+8 and so on. And not look for unknown library constructs, since this might be in conflict with rules 1 and 2.

Edit: Also output the found barcodes to a separate file

@remiolsen remiolsen self-assigned this Oct 6, 2020
@remiolsen remiolsen added this to the 0.4.0 milestone Oct 6, 2020
@remiolsen remiolsen mentioned this issue Jul 19, 2021
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

Successfully merging a pull request may close this issue.

2 participants