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It would be nice to be able to supply a file of all known illumina indexes to screen for unexpected contamination, even for low level pool contamination.
The text was updated successfully, but these errors were encountered:
Suggestion after discussing this with @FranBonath is to do a count of barcodes using the same mapping procedure that already exists, with the following rules:
Only use perfectly mapping barcodes (i.e. no indels)
Exclude i5+i7 barcodes found in the samplesheet
Output a list of 100 (500?) most abundant barcodes found
At first, use only the library constructs that are in the samplesheet. For instance single-index 8bp separately, from dual index 8+8 and so on. And not look for unknown library constructs, since this might be in conflict with rules 1 and 2.
Edit: Also output the found barcodes to a separate file
It would be nice to be able to supply a file of all known illumina indexes to screen for unexpected contamination, even for low level pool contamination.
The text was updated successfully, but these errors were encountered: