diff --git a/README.md b/README.md index 2b89a7f..944f6fe 100644 --- a/README.md +++ b/README.md @@ -415,7 +415,9 @@ Here the description of typical ouput you will get from AliNe: In order to compare several aligner output you should activate the `--fastqc` parameter. AliNe will run the FastQC program for each output file, thereafter all FastQC file will be gathered in an html file using MultiQC. The resulting file called `multiqc_report.html` can be found in /MultiQC ( by default is called `alignment_results` and can be setup using `--outdir` AliNe parameter). -FastQC collect the following information: +To compare the output of multiple aligners, you should enable the `--fastqc` parameter. AliNe will execute the FastQC program for each output file. Subsequently, all FastQC reports will be gathered into an HTML file using MultiQC. The resulting file, named `multiqc_report.html`, can be found in the `/MultiQC directory`. By default, the output directory is named `alignment_results`, but this can be customized using the `--outdir` parameter in AliNe. + +FastQC collects the following information: * Sequence Counts * Sequence Quality * Per Sequence Quality Scores @@ -429,7 +431,7 @@ FastQC collect the following information: #### General Statistics -Sequence Duplication Levels, Per Sequence GC Content and Sequence Counts are reported at the top of the `multiqc_report.html` file in a table called `General Statistics` as % Dups, %GC a,d M Seqs accordingly (see below). +"Sequence Duplication Levels", "Per Sequence GC Content" and "Sequence Count" are reported at the top of the `multiqc_report.html` file in a table called `General Statistics` as "% Dups", "%GC" and "M Seqs" accordingly (see below).